Cell culturing methods and apparatus

Details Cell culturing methods and apparatus Cell culturing methods and apparatus

1984-06-21

1988-03-29

Scott, Samuel

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

435287, 435 4, 435 29, 436 4, 436 34, 422 48, 422 61, 422 68, C12Q 129, C12Q 300

Patent

active

047343729

ABSTRACT:
A system and protocol for performing cytotoxicity studies including apparatus and methods for culturing the biopsied cells, for providing oxygenated nutrients, for removing cell-waste products, for introducing a fluorogenic substrate, for introducing cytotoxic agents including anticancer drugs, for measuring the released fluorescence and for measuring intracellular accumulation of fluorescein. Cytotoxicity can be determined by measuring fluorescence in the efflux of the vessel and concommitantly by direct photometric comparisons of the cells in the vessel before and after exposure to the cytotoxic agent.

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