Cell-culturing apparatus and method employing a macroporous supp

Details Cell-culturing apparatus and method employing a macroporous supp Cell-culturing apparatus and method employing a macroporous supp

1992-07-29

1993-11-16

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352401, 4352402, 43524024, 435283, 435284, 435285, 435286, 435287, 435313, 435314, 435315, 435316, C12N 500, A01N 102, C12M 300

Patent

active

052623203

ABSTRACT:
An apparatus and method are disclosed for culturing cells by flowing a medium through a packed bed of biocompatible macroporous ceramic particles disposed in a cell-culture reactor. In one embodiment, oxygen and other nutrients in the medium are transported significantly by convective flow through pores of the biocompatible macroporous ceramic particles to cells disposed within the pores. Oxygen can be introduced to the cell-culture reactor by sparging oxygen gas into the cell-culture reactor. Oxygen content in the medium can also be increased by increasing the solubility of oxygen in the medium, such as by adding perfluorocarbon or other oxygen carriers to the medium. The oxygen capacity of the reactor can be further increased by sparging oxygen-containing gas into the medium at a plurality of points in the packed bed, or by introducing oxygen gas to the medium through a solid-phase oxygen gas-permeable membrane disposed in the packed bed. Alternatively, an oxygen-containing gas can be directed through an oxygen-permeable conduit disposed within at least one passage of a biocompatible macroporous support.

REFERENCES:
patent: 4153510 (1979-05-01), Messing et al.
patent: 4537860 (1985-08-01), Tolbert et al.
patent: 4757017 (1988-07-01), Cheung
patent: 4789634 (1988-12-01), Muller-Lierheim et al.
patent: 4833083 (1989-05-01), Saxena
patent: 4919895 (1990-04-01), Heldebrant et al.
patent: 4948728 (1990-08-01), Stephanopoulos et al.
Racher et al., "Studies on Monoclonal Antibody Production By A Hybridoma Cell Line (C1E3) Immobilized in a Fixed Bed, Porosphere Culture System," Eng. Conf. on Cell Culture at Santa Barbara, Calif., (Dec. 3-8, 1989).
Stephanopoulos et al., "The Effect of Intraparticle Convection on Nutrient Transport in Porous Biological Pellets", Chem. Eng. Science, 44, 2031-2039 (1989).
Tsiveriotis, "Convection Inside a Porous Microbial Particle As A Means of Nutrient Transport", Masters' Thesis, Massachusetts Institute of Technology (1988).
Stephanopoulos, et al., "Porous Microcarriers for Cell Culture", 9:74 (1989).
Lyderson, "Perfusion Cell Culture System Based on Ceramic Matrices", 170-192 (1987).

Affiliated with

Also associated with

Rating

Cell-culturing apparatus and method employing a macroporous supp does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cell-culturing apparatus and method employing a macroporous supp, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cell-culturing apparatus and method employing a macroporous supp will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-21819