Cell culture vessel and cultured cell

Details Cell culture vessel and cultured cell Cell culture vessel and cultured cell

2004-11-17

2010-02-02

Griffin, Walter D (Department: 1797)

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

C435S283100, C435S309400

Reexamination Certificate

active

07655457

ABSTRACT:
It is an object of this invention to provide a cell culture vessel which has a simple and convenient structure, can prevent the injury on the cells at the time of peeling, and accelerates the transportation of nutrients and discharge of waste materials. In order to solve the problem mentioned above, the cell culture vessel of this invention has protrusions having a corresponding diameter not smaller than 10 nm and not greater than 10 μm and a height not smaller than 10 nm and not greater than 1 mm on the surface of the cell culture vessel. The protrusions make the culture fluid permeate into the under part of the cells, accelerate the supply of nutrients and discharge of waste materials, and makes the contact between the cells and the vessel as a point-contact and thereby prevents the cells from the injury which the cells undergo at the time of peeling.

REFERENCES:
patent: 4761378 (1988-08-01), Godsey
patent: 4975377 (1990-12-01), Key
patent: 5084393 (1992-01-01), Rogalsky
patent: 5264344 (1993-11-01), Sneath
patent: 6852525 (2005-02-01), Cantor
patent: 7195872 (2007-03-01), Agrawal et al.
patent: 85 1 03552 (1986-10-01), None
patent: WO 00/37606 (2000-06-01), None

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