Cell-culture reactor having a porous organic polymer membrane

Chemistry: molecular biology and microbiology – Apparatus – Bioreactor

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Details

4352971, 4352992, 4353042, 4353043, C12M 306

Patent

active

060748710

ABSTRACT:
A method for making a biocompatible polymer article using a uniform atomic oxygen treatment is disclosed. The substrate may be subsequently optionally grated with a compatibilizing compound. Compatibilizing compounds may include proteins, phosphorylcholine groups, platelet adhesion preventing polymers, albumin adhesion promoters, and the like. The compatibilized substrate may also have a living cell layer adhered thereto. The atomic oxygen is preferably produced by a flowing afterglow microwave discharge, wherein the substrate resides in a sidearm out of the plasma. Also, methods for culturing cells for various purposes using the various membranes are disclosed as well. Also disclosed are porous organic polymers having a distributed pore chemistry (DPC) comprising hydrophilic and hydrophobic regions, and a method for making the DPC by exposing the polymer to atomic oxygen wherein the rate of hydrophilization is greater than the rate of mass loss.

REFERENCES:
patent: 4921612 (1990-05-01), Sirkar
patent: 5141806 (1992-08-01), Koontz
patent: 5215790 (1993-06-01), Koontz
patent: 5332551 (1994-07-01), Koontz
patent: 5369012 (1994-11-01), Koontz et al.

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