Cell culture process and medium for the growth of adherent anima

Details Cell culture process and medium for the growth of adherent anima Cell culture process and medium for the growth of adherent anima

1994-12-13

1999-02-16

Rollins, John W.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352401, 4352402, 4352403, C12N 500

Patent

active

058719997

ABSTRACT:
Adherent animal cells are grown in suspension in a nutrient medium in which the molar ratio of total inorganic ions and total amino acids is reduced when compared with the corresponding ratio in known nutrient media, and is maintained at a level at which little or no cell aggregation occurs. Further, the nutrient medium contains 10:1 to about 1:1 molar ratio of total inorganic ions to total amino acids. In one further embodiment, the nutrient medium contains a total sodium ion concentration in the range of 75 to 120 mM (millimole), a total chloride ion concentration in the range of 50 to 90 mM and a total amino acid content of 20 to 50 mM. The adherent animal cells include mammalian cells such as cells belonging to a human, rat, mouse or hamster. Chinese Hamster Ovary Cells (CHO cells) are cultured using the process of the disclosed invention.

REFERENCES:
patent: 3850748 (1974-11-01), Cook et al.
patent: 4124448 (1978-11-01), Narasimhan et al.
patent: 5122469 (1992-06-01), Mather et al.
Brown et al, "On-Line Removal Of Cells From Continuous Suspension Cultures", pp. 416-420, (1991).
Goetghebeur et al, "Microsphere-Induced Aggregate Culture Of Animal Cells", pp. 423-428.
Litwin, "The Growth of Cho And BHK Cells As Suspended Agregates In Serum-Free Medium", pp. 429-433.
Ham et al, "Media And Growth Requirements", Methods In Enzymology, vol. 58:44-93, (1979).
Field et al, "Production Of A Chimeric Antibody For Tumour Imaging And Therapy From Chinese Hamster Ovary (CHO) And Myeloma Cells", pp. 742-744.

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