Cell culture process

Details Cell culture process Cell culture process

1976-11-03

1977-11-22

Rosen, Sam

Textiles: fiber preparation

C12K 900

Patent

active

040594866

ABSTRACT:
SV3T3 cells are rapidly propagated in vitro to elaborate MIF and TAF by first growing on a surface and thereafter transferring an inoculum of the growing cells into an agitated liquid suspension of nutrient culture medium, incubating at 30.degree.-38.degree. C while continuing the agitation of the medium, periodically transferring the growing cells into fresh medium and continuing the incubation with agitation of the medium, whereby said cells are proliferated in aggregates and elaborate MIF and TAF in significantly detectable amounts.

REFERENCES:
patent: 3850748 (1974-11-01), Cook et al.
Gottlieb et al.--Fundamental Aspects of Neoplasia (1975), pp. 401-412.
Hammond et al.--Science, vol. 185 (Sept. 13, 1974), pp. 955-957.
Otsuka et al.--J. of Cell Physiology, vol. 85, (1975), pp. 665-674.
Otsuka et al.--J. of Cell Physiology, vol. 87, (1975), pp. 213-220.
Renger et al.--J. of Virology, vol. 11, No. 5 (1973), pp. 702-708.
Gail et al.--Exper. Cell Research, vol. 70, (1972), pp. 33-40.
Ohman et al.--Proc. Nat. Acad. Science, vol. 70, No. 5, (1973), pp. 1569-1573.
Willmer--Cells & Tissues in Culture, vol. 1, (1965), pp. 495 & 496.

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