Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2008-05-12
2010-11-09
Robinson, Hope A (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S252000, C435S325000, C435S007100
Reexamination Certificate
active
07829309
ABSTRACT:
This invention relates generally to the field of cell culture. More particularly, the invention relates to improving viability of recombinant cell cultures and the yields of secreted polypeptides therefrom by the addition of betaine to the tissue culture medium.
REFERENCES:
patent: 5122469 (1992-06-01), Mather et al.
patent: 5633162 (1997-05-01), Keen et al.
patent: 5705364 (1998-01-01), Etcheverry et al.
van der Heide et. al., EMBO J., vol. 20, No. 24, pp. 7022-7032, 2001).
Urlaub et al., “Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity” Proc. Natl. Acad Sci USA 77:4216-4220 (1980).
Rasmussen et al., “Isolation, characterixation and recombinant protein expression in Veggie-CHO: A serum-free CHO host cell line,” Cytotechnology, 28:31-42 1998.
Kim et al., “Osmoprotective Effect of Glycine Betaine on Thrombopoietin Production in Hyperosmotic Chinese Hamster Ovary Cell Culture Clonal Variations,” Biotechnol Prog 16:775-781 (2000).
Ryu et al., “Osmoprotective Effect of Glycine Betaine on Foreign Protein Production in Hyperosmotic Recombinant Chinese Hamster Ovary Cell Cultures Differs Among Cell Lines,” Biotech Bioeng., 70:167-175 (2000).
Petronini et al., “Modulation by Betaine of Cellular Responses to Osmotic Stress,” Biochem J. 15;282 (Pt 1) 69-73 (1992).
Follstad Brian D.
Potter Anne H.
Bellas Christine M.
Fowler Kathleen
Immunex Corporation
Robinson Hope A
LandOfFree
Cell culture performance with betaine does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Cell culture performance with betaine, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cell culture performance with betaine will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4250265