Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2006-06-27
2006-06-27
Weber, Jon (Department: 1653)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S320100, C530S410000
Reexamination Certificate
active
07067279
ABSTRACT:
This invention relates generally to the field of cell culture. More particularly, the invention relates to improving viability of recombinant cell cultures and the yields of secreted polypeptides therefrom by the addition of betaine to the tissue culture medium.
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Petronini et al., Modulation by betaine of cellular responses to osmotic stress, Biochem J. Feb. 15, 1992;282 (Pt 1):69-73.
Urlaub et al., Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activityProc Natl Acad Sci USA 77:4216-4220 (1980).
Rasmussen et al., Isolation, characterization and recombinant protein expression in Veggie-CHO: A serum-free CHO host cell lineCytotechnology, 28:31-42 (1998).
Kim et al., Osmoprotective Effect of Glycine Betaine on Thrombopoietin Production in Hyperosmotic Chinese Hamster Ovary Cell Culture Clonal VariationsBiotechnol Prog., 16:775-781 (2000).
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Follstad Brian D.
Potter Anne H.
Immunex Corporation
Marshall & Gerstein & Borun LLP
Mondesi Robert B.
Weber Jon
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