Cell culture model for drug bioavailability

Details Cell culture model for drug bioavailability Cell culture model for drug bioavailability

1997-01-07

1999-01-05

Scheiner, Toni R.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of regulating cell metabolism or physiology

435366, 435370, 435352, 435384, 435363, C12N 506, C12N 508, C12N 138

Patent

active

058561897

ABSTRACT:
A model utilizing cells for assessing oral bioavailability and potential drug-drug interactions of pharmacological agents is described. The model subjects cells (e.g., Caco-2 cells) to conditions that result in reliable expression of catalytically-active CYP3A4 at levels that appear to be comparable to levels present in mature enterocytes. These conditions include plating of selected clones on an extracellular matrix, exposure to 1.alpha.,25-(OH).sub.2 -D.sub.3 for a defined period of time, and the presence of serum in the medium. The model is useful for defining the role of CYP3A4 in limiting the oral bioavailability of many pharmacological agents and in drug-drug interactions involving CYP3A4 substrates that are believed to occur largely at the level of the intestine.

REFERENCES:
Cross, et al., Naunyn-Schmiedeberg's Arch. Pharmacol., vol. 347, pp. 105-110, 1993.
Schmiedlin-Ren, et al., Molecular Pharmacology, vol. 51, pp. 741-754, 1997.
Riley, et al., Biochim. Biophys. Acta, vol. 1066, No. 2, pp. 175-182, Jul. 1991, Abstract Only.

Affiliated with

Also associated with

Rating

Cell culture model for drug bioavailability does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cell culture model for drug bioavailability, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cell culture model for drug bioavailability will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-861629