Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1979-07-02
1981-10-06
Rosen, Sam
Chemistry: molecular biology and microbiology
Spore forming or isolating process
525 61, 525379, 536 18, 536 30, 536 45, 536 51, 536112, C12N 502, C08B 3700
Patent
active
042936543
ABSTRACT:
Improved cell culture microcarriers, and methods for their production and use, are disclosed herein. These improved microcarriers have positive charge capacities adjusted and/or controlled within a range suitable for good cell growth. One method for producing such improved microcarriers is by treating beads formed from polymers containing pendant hydroxy groups, such as dextran beads, with an aqueous solution of an alkaline material and a chloro- or bromo-substituted tertiary amine under precisely controlled conditions to produce the desired exchange capacity. The resultant positively charged microcarriers have been used in microcarrier cultures to produce outstanding growth of anchorage-dependent cells. Such cells can be harvested, or used for the production of viruses, vaccines, hormones, interferon or other cellular growth by-products.
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Horng-Primary Culture of Mammalian Cells on Microcarrier Surface-Ph.D. Thesis, State University of N.Y. Buffalo 1975.
Levine et al.-Proc. 1st Internat. Cell Culture Congress (Sep. 21-25, 1975) Birmingham, AL.
Levine David W.
Thilly William G.
Wang Daniel I. C.
Wong Jason S.
Brook David E.
Massachusetts Institute of Technology
Rosen Sam
Smith, Jr. Arthur A.
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