Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1992-03-03
1994-08-30
Hill, Jr., Robert J.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435 4, 4351723, 4352402, 435948, C12N 100, C12N 500, C12N 1500, C12Q 100
Patent
active
053427777
ABSTRACT:
The present invention relates to cell medium developed to support long term multiplication and permanent establishment of a cell line of human liver epithelial cells. The medium may contain an effective cell growth promoting amount of calcium ions; an effective cell growth promoting amount of glucose; an effective amount of insulin to aid cells in glucose uptake; an effective cell growth promoting amount of hydrocortisone; an effective amount of epidermal growth factor to bind epidermal growth factor receptors on cells; an effective amount of transferrin to increase DNA synthesis in cells; an effective amount of cholera toxin to increase DNA synthesis in cells; an effective amount of triiodothyronine to increase DNA synthesis in cells; and an effective growth promoting amount of mammalian hormones and mitogenic factors, including lipoprotein, cholesterol, phospholipids and fatty acids.
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Cole Katharine H.
Harris Curtis C.
Lechner John F.
Hill Jr. Robert J.
The United States of America as represented by the Secretary of
Wang Gian P.
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