Cell culture media for enhanced protein production

Details Cell culture media for enhanced protein production Cell culture media for enhanced protein production

2002-10-04

2009-10-13

Afremova, Vera (Department: 1657)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of culturing cells in suspension

C435S375000, C435S404000

Reexamination Certificate

active

07601535

ABSTRACT:
Culture media for in vitro cell culture which contain substantially saturated amounts of selected amino acids improve protein production, constrain cell growth and extend cell longevity, methods for the production and use of such media, and systems for the production of protein utilizing such media and methods.

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Chua et al. “Enhanced IgG production in eRDF media with and without serum”. Journal of Immunological Methods. 1994. 167:109-119.
Chua, Florence K.F. et al., “Hyper-stimulation of monoclonal antibody production by high osomlarity stress in eRDF medium,”J. Biotechnologyvol. 37, pp. 265-275, 1994.
Murakami, Hiroki, “Serum-Free Media Used for Cultivation of Hybridomas”,Monoclonal Antibodies: Production and Application, pp. 107-141, 1989.
Oh, Steve K.W. et al., “Intracellular Responses of Productive Hybridomas Subjected to High Osmotic Pressure”,Biotechnology and Bioengineering, vol. 46, pp. 525-535, 1995.

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