Cell culture-containing tubular capsule produced by co-extrusion

Details Cell culture-containing tubular capsule produced by co-extrusion Cell culture-containing tubular capsule produced by co-extrusion

1991-01-08

1994-02-01

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435179, 43524022, 43524023, C12N 1104, C12N 1112, C12N 500

Patent

active

052831876

ABSTRACT:
Living cells such as animal cells which produce biologically active factors are encapsulated within a semipermeable, polymeric membrane such as polyacrylate by co-extruding an aqueous cell suspension and a polymeric solution through a common port having at least one concentric bores to form a tubular extrudate having a polymeric membrane which encapsulates the cell suspension. The cell suspension is extruded through an inner bore and the polymeric solution is extruded through an outer bore while a pressure differential is maintained between the cell suspension and the polymeric solution to impede solvent diffusion from the polymeric solution into the cell suspension. The polymeric solution coagulates to form an outer coating or membrane as the polymeric solution and the cell suspension are extruded through the extrusion port. As the outer membrane is formed, the ends of the tubular extrudate are sealed to form a cell capsule. In one embodiment, the tubular extrudate is sealed at intervals to define separate cell compartments connected by polymeric links. In another embodiment, a cell capsule connected to a tethering filament is formed. The polymeric membrane may contain additives such as a surfactant, an anti-inflammatory agent or an anti-oxidant and can be coated with a protective barrier. The cell suspension may contain nutrients and an anchorage substrate.

REFERENCES:
patent: 3093831 (1963-06-01), Jordan
patent: 3715277 (1973-02-01), Dinelli et al.
patent: 3821087 (1974-06-01), Knazek et al.
patent: 3947325 (1976-03-01), Dinelli et al.
patent: 3962383 (1976-06-01), Hagiwara et al.
patent: 4163691 (1979-08-01), Devos et al.
patent: 4241187 (1980-12-01), White
patent: 4251195 (1981-02-01), Suzuki et al.
patent: 4324683 (1982-04-01), Lim et al.
patent: 4333906 (1982-06-01), Porter et al.
patent: 4352883 (1982-10-01), Lim
patent: 4353888 (1982-10-01), Sefton
patent: 4378016 (1983-03-01), Loeb
patent: 4391909 (1983-07-01), Lim
patent: 4402694 (1983-09-01), Ash et al.
patent: 4409331 (1983-10-01), Lim
patent: 4426337 (1984-01-01), Suzuki et al.
patent: 4451253 (1984-05-01), Harman
patent: 4495288 (1985-01-01), Jarvis, Jr. et al.
patent: 4686098 (1987-08-01), Kopchick et al.
patent: 4695466 (1987-09-01), Morishita et al.
patent: 4902295 (1990-02-01), Walthall et al.
patent: 4902450 (1990-02-01), Morrison
Schratter (1976) in Methods in Cell Biology, Academic Press, N.Y., vol. 14, pp. 95-103.
Tze et al. (1976) Nature 264:466-467.
Chang (1977) in Biomedical Applications of Immobilized Enzymes and Protein, Plenum Press, N.Y., vol. 1, pp. 69-90.
Sun et al. (1977) Diabetes 26:1136-1139.
Sugamori et al. (1989) Trans Am Soc Artif Intern Organs XXXV:791-799.
Sefton et al. (1987) Biotech and Bioeng XXIX:1135-1143.
Tresco et al. (1992) ASAIO Journal 38:17-23.
Aebischer et al. (1991) Science 242:133.
Winn et al. (1991) Experimental Neurology 113: 322-329.
Aebischer et al. (1991) Brain Research 560:43-49.
Aebischer et al. (1991) J. Biomec. Engineering 113:178-183.
Aebischer et al. (1991) Experimental Neurology 111:269-275.
Aebischer et al. (1991) Biomaterials 12:50-56.
Jaeger et al. (1991) Brain Research 551: 163-170.
Hoffman et al. (1990) Experimental Neurology 110:39-44.
Winn et al. (1989) Experimental Neurology 105:244-250.
Winn et al. (1989) J. Biomed. Mater. Res. 23:31-44.
Aebischer et al. (1988) Brain Research 448:364-368.
Jaeger et al., Progress in Brain Research, vol. 82, 1990, pp. 41-46.

Affiliated with

Also associated with

Rating

Cell culture-containing tubular capsule produced by co-extrusion does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cell culture-containing tubular capsule produced by co-extrusion, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cell culture-containing tubular capsule produced by co-extrusion will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-579447