Cell culture container that self-seals after cannula penetration

Details Cell culture container that self-seals after cannula penetration Cell culture container that self-seals after cannula penetration

1997-04-09

1999-07-27

Naff, David M.

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

424 937, 4352831, 435394, 435395, C12M 300, C12N 500, C12N 506, A01N 6300

Patent

active

059289368

ABSTRACT:
A container for growing cells in a culture medium is made by sealing two sheets of gas-permeable elastomeric material such as made of silicone together to enclose a culture chamber. A portion of one of the sheets is in the shape of a dome having a wall extending transverse to a major plane of that sheet. The thickness of the wall is greater than the thickness of the rest of the sheet. A permeable and porous body, preferably coated with a thin film of metal, may be in the culture chamber to provide a substrate for three-dimensional growth. A porous body can be implanted in an organ of an animal to allow dividing cells to grow into the porous body. The porous body is removed from the animal and placed in contact with other porous bodies in the culture chamber where cells grow from the porous body containing cells into the other porous bodies. Thereafter, the chamber can be opened and each body with cells combined with additional porous bodies so that additional cells can be grown to provide an almost unlimited source of cells from having started with the organ of only one animal.

REFERENCES:
patent: 4224413 (1980-09-01), Burbidge
patent: 4496362 (1985-01-01), Leurink
patent: 4604360 (1986-08-01), Hounsell
patent: 5282861 (1994-02-01), Kaplan
patent: 5374264 (1994-12-01), Wadsworth, Jr.
patent: 5523228 (1996-06-01), Ingram et al.
patent: 5620420 (1997-04-01), Kriesel
Tantalum Foams for Cancellous Bone Implants, dated Mar. 1993 by Richard B. Kaplan, et al.
Canine Studies of Bone Ingrowth with Implex Corporation Tantalum Porous Implants, Progress Report, dated Jun. 27, 1995, by J.D. Bobyn, Ph.D., et al.

Affiliated with

Also associated with

Rating

Cell culture container that self-seals after cannula penetration does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cell culture container that self-seals after cannula penetration, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cell culture container that self-seals after cannula penetration will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-877948