Cell culture bioreactor

Details Cell culture bioreactor Cell culture bioreactor

1993-07-22

1995-08-22

Beisner, William H.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524024, 435286, 435313, C12N 502, C12M 302

Patent

active

054439850

ABSTRACT:
The invention provides a bioreactor for culturing living cells, particularly shear sensitive cells, wherein the bioreactor is composed of a stationary vessel with opposite spaced walls inclined at an angle to form upper and lower walls. Liquid culture medium and cell culture, such as hybridoma cells, are introduced into the vessel and gas is introduced at the lower end of the vessel to form gas bubbles which travel upward along the upper wall of the bioreactor to disengage from a small portion of the gas liquid interface. The gas bubbles circulate the cells and liquid medium, maintaining the cells in suspension and lifting them in a circulating path upwardly parallel to the upper wall and downward along the lower wall. The bioreactor design thus achieves bulk mixing and aeration by maintaining a significant degree of segregation between the upwardly travelling bubbles and the cells in the liquid medium avoiding unnecessary cell damage by fluid-mechanical shear or by bubble bursting events.

REFERENCES:
patent: 1019003 (1912-02-01), Aminoff
patent: 1983058 (1934-12-01), Wait
patent: 3402103 (1968-09-01), Amberg et al.
patent: 3506541 (1970-04-01), Snelling
patent: 3575813 (1971-04-01), Rothmayr
patent: 4228242 (1980-10-01), Girard et al.
patent: 4343904 (1982-08-01), Birch et al.
patent: 4649117 (1987-03-01), Famiuetti
patent: 4898718 (1990-02-01), Cardoso
patent: 4906577 (1990-03-01), Armstrong et al.
patent: 5057428 (1991-10-01), Mizutani et al.
patent: 5057429 (1991-10-01), Watanabe et al.

Affiliated with

Also associated with

Rating

Cell culture bioreactor does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cell culture bioreactor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cell culture bioreactor will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2141311