Cell cultivation method and floating animal cell culture unit fo

Details Cell cultivation method and floating animal cell culture unit fo Cell cultivation method and floating animal cell culture unit fo

1980-09-18

1983-07-05

Jones, Raymond N.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435284, C12N 502, C12M 300

Patent

active

043919120

ABSTRACT:
A method of cultivating floating animal cells, such as lymphocytic cells, medullary tumor cells, medullary tumor cells obtained by the cell fusion with other cells, lymphoma cells, leukemic cells, leukocytic cells, mastocarcinoma cells, liver cancer cells, etc., which comprises introducing a culture medium in a cell culture unit comprising a shell and a plurality of hollow fibers enclosed in the shell, said hollow fiber being open at either end outside of the shell and having a pore diameter of from about 20 A to 10.sup.5 A, wherein the culture medium passes through the interior of the hollow fibers and the floating animal cells are cultivated in the space between the shell and the hollow fibers is disclosed. A floating animal cell culture unit for the method is also disclosed.

REFERENCES:
patent: 3734851 (1973-05-01), Matsumura
patent: 3821087 (1974-06-01), Knazek et al.
patent: 3883393 (1975-05-01), Knazek et al.
patent: 3911140 (1975-10-01), Osborne et al.
patent: 4087327 (1978-05-01), Feder et al.
patent: 4201845 (1980-05-01), Feder et al.
patent: 4242460 (1980-12-01), Chick et al.
Ham, Richard G. et al., "Media and Growth Requirements"; Methods in Enzymology, Jakoby, W. R. et al., eds., vol. 58, `Cell Culture`; Academic Press, 1979; pp. 44-49, 81 and 82.

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