Cell cultivating apparatus

Details Cell cultivating apparatus Cell cultivating apparatus

1991-09-30

1993-02-23

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Apparatus

Mutation or genetic engineering apparatus

435284, 435289, 435311, 435315, 21032167, 21032169, C12M 304, C12M 306

Patent

active

051889625

ABSTRACT:
Culture medium is circulated from a culture medium tank, through a first main duct, through a cell cultivating vessel containing therein a bundle of hollow threads, through a second main duct and back to the culture mediun tank. The first main duct is provided at an intermediate point with a vibrating columnar pump adapted to pulsate the culture medium and to vibrate outer surfaces of said hollow threads. The second main duct is provided along a tail end portion with a container serving to capture solids in the culture medium. The first main duct branches into a first sub-duct which is provided with sensors used to detect and regulate a quantity of gas mixture in the culture medium. Similarly, the second main duct branches into a second sub-duct which is provided with sensors used to detect a quantity of gas mixture in the culture medium which has been used for cell cultivation so that a level of cell metabolism occurring in the hollow threads may be detected and a flow velocity of the culture medium may be correspondingly regulated.

REFERENCES:
patent: 2490443 (1949-12-01), Knipper
patent: 2761392 (1956-09-01), Parker
patent: 3460680 (1969-08-01), Domnick
patent: 3606595 (1971-09-01), Takamizawa
patent: 4889812 (1989-12-01), Guinn et al.
patent: 4999298 (1991-03-01), Wolfe et al.
Seaver, Commercial Production of Monocolonal Antibodies, N.Y., Marcel Dekker Inc., 1987, pp. 119-138.

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