Cell control used to confirm enzymatic activity

Details Cell control used to confirm enzymatic activity Cell control used to confirm enzymatic activity

1996-10-29

1999-10-19

Weber, Jon P.

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

436 63, 436172, 435 4, 435 18, 435 405, 435372, G01N 3100, C12Q 100, C12Q 134, C12N 508

Patent

active

059688319

ABSTRACT:
The invention provides a cell control product for use with a cytoenzymology assay to confirm enzymatic activity in devices employing electronic and/or optical means. The cell control product comprises a lyophilized mammalian cell which is capable of being rehydrated in water to exhibit cellular structure and cellular enzymatic activity so that said lyophilized cells can function effectively as a cytoenzymology cell control in an enzymatic analysis. The cellular structure of the cell control is capable of being authenticated by light scatter or microscopy analysis and the cellular enzymatic activity is capable of being authenticated by fluorescence analysis. The cell control exhibits a real time stability when stored at between 2 and 8.degree. C. for at least a two month period of time. Preferably the mammalian cell is an abnormal cell selected from Molt 4, CCRF-CEM and HL-60 cell lines.

REFERENCES:
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patent: 5178884 (1993-01-01), Goodrich et al.
patent: 5443986 (1995-08-01), Haughland et al.
Dolbeare, Modern Fluorescence Spectroscopy 3, ed., E.L. Wehry, Plenum Press, NY, Chapter 6, pp. 251-293, 1981.
Lakowicz, Principles of Fluorescence Spectroscopy, Plenum Press, NY, Chapter 1, pp. 1 and 15-18, and Chapter 2, pp. 19 and 20, 1983.
ATCC Catalogue of Cell Lines and Hybridomas, 6th Edition, pp. 69, 70, 132, and 152, 1988.

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