Cell based signal generation

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S243000, C435S254100, C435S254110, C435S254200, C435S254210, C435S255200

Reexamination Certificate

active

07022513

ABSTRACT:
The present invention makes available a rapid, reproducible, robust assay system for screening and identifying pharmaceutically effective compounds that specifically interact with and modulate the activity of a cellular protein, e.g., a receptor or ion channel. The subject assay enables rapid screening of large numbers of compounds to identify those which act as an agonist or antagonist to the bioactivity of the cellular protein. In particular, the assay of the invention makes use of a cell that harbors a protein that is responsive to a cellular signal transduction pathway. The protein is operatively linked to a polypeptide which causes a detectable signal to be generated upon stimulation of the pathway, e.g., when a compound interacts with and modulates the activity of a cellular receptor or ion channel of the cell. Thus, the cell provides a signal generation means comprising a novel fusion protein the expression of which is independent of stimulation/activation of the signal transduction pathway, but the activity of which is responsive to the signal transduction pathway.

REFERENCES:
patent: 4418149 (1983-11-01), Ptashne et al.
patent: 4833080 (1989-05-01), Brent et al.
patent: 4948874 (1990-08-01), Kronvall et al.
patent: 5096815 (1992-03-01), Ladner et al.
patent: 5283173 (1994-02-01), Fields et al.
patent: 5401629 (1995-03-01), Harpold et al.
patent: 5436128 (1995-07-01), Harpold et al.
patent: 5468614 (1995-11-01), Fields et al.
patent: 5482835 (1996-01-01), King et al.
patent: 5580736 (1996-12-01), Brent et al.
patent: 5691188 (1997-11-01), Pausch et al.
patent: 5739029 (1998-04-01), King et al.
patent: 5789184 (1998-08-01), Fowlkes et al.
patent: 568925 (1993-11-01), None
patent: WO 88/10308 (1988-12-01), None
patent: WO 91/12273 (1991-08-01), None
patent: WO 92/05244 (1992-04-01), None
patent: WO 92/08740 (1992-05-01), None
patent: WO 93/10230 (1993-05-01), None
patent: WO 94/23025 (1994-10-01), None
patent: WO 95/30012 (1995-11-01), None
patent: WO 97/11159 (1997-03-01), None
patent: WO 98/13513 (1998-04-01), None
Akada, R. et al. “Genetic Relationships Between the G Protein βγ Complex, Ste5p, Ste20p and Cdc42p: Investigation of Effector Roles in the Yeast Pheromone Response Pathway,”Genetics143:103-117 (1996).
Alison, Malcolm R. et al. “Growth factors and growth factor receptors,”Brit. J. of Hosp. Med.49(11):774-88 (1993).
Altieri, Dario C. “Proteases and protease receptors in modulation of leukocyte effector functions,”J. of Leukocyte Biol.58:120-27 (1995).
Artemyev, Nikolai O. et al. “Sites of Interaction between Rod G-Protein α-Subunit and cGMP-phosphodiesterase γ-Subunit,”J. Biol. Chem.267(35):25067-72 (1992).
Awramik, S. M. “New fossil finds in old rocks,”Nature319:446-47 (1986).
Belka, C. et al. “The role of tyrosine kinases and their substrates in signal transmission of hematopoietic growth factors: a short review,”Leukemia9:754-61 (1995).
Bender, Alan and Sprague,George F. Jr. “Pheromones and Pheromone Receptors Are the Primary Determinants of Mating Specificity in the YeastSaccharomyces cerevisiae,” Genetics121:463-76 (1989).
Birnbaumer, Lutz “Transduction of receptor signal into modulation of effector activity by G proteins: the first 20 years or so . . . ”FASEB Journal4:3178-88 (1990).
Blinder, Dmitry et al. “Constitutive Mutants in the Yeast Pheromone Response: Ordered function of the Gene Products,”Cell56:479-486 (1989).
Brill, Julie A. et al. “A Role for Autophosphorylation Revealed by Activated Alleles ofFUS3, the Yeast MAP Kinase Homolog,”Molecular Biology of the Cell5:297-312 (1994).
Brugarolas, James et al. “Radiation-induced cell cycle arrest compromised by p21 deficiency,”Nature377:522-57 (1995).
Burack, W. Richard et al. “The Activating Dual Phosphorylation of MAPK by MEK Is Nonprocessive,”Biochemistry36(20):5929-5933 (1997).
Cavallini, Bruno et al. “A yeast activity can substitute for the HeLa Cell TATA box factor,”Nature334:77-80 (1988).
Chambers, D. A. et al. “Neuroimmune Modulation: Signal Transduction and Catecholamines,”Neurochem. Int. 22(2):95-110 (1993).
Chan, Russell K. and Otte, Carol A. “Isolation and Genetic Analysis ofSaccharomyces cerevisiaeMutants Supersensitive to G1 Arrest by a Factor and α Factor,”Molecular and Cellular Biol. 2(1):11-20 (1982).
Chang, Fred and Herskowitz, Ira “Identification of a Gene Necessary for Cell Cycle Arrest by a Negative Growth Factor of Yeast: FAR1 is an Inhibitor of a G1 Cyclin, CLN2,”Cell63:999-1011 (1990).
Chien, Cheng-Ting, et al. “The two-hybrid system: A method to identify and clone genes for proteins that interact with a protein of interest,”Proc. Natl. Acad. Sci. USA 88:9578-82 (1991).
Clark, Karen L. et al. “Interactions among the Subunits of the G-protein Involved inSaccharomyces cerevisiaeMating,”Molecular and Cellular Biol. 13(1):1-8 (1993).
Cole, Gary M. et al. “Stoichiometry of G Protein Subunits Affects theSaccharomyces cerevisiaeMating Pheromone Signal Transduction Pathway,”Molecular and Cellular Biology10(2):510-517 (1990).
Coleman, David E. et al. “Structures of Active Conformation of Gia1and the Mechanism of GTP Hydrolysis,”Science265:1405-12 (1994).
Conklin, Bruce R. et al. “Substitution of three amino acids switches receptor specificity of Gqato that Gia1”Nature363:274-76 (1993).
Cwirla, Steven E. et al. “Peptides on phage: A vast library of peptides for identifying ligands,”Proc. Natl. Acad. Sci. USA87:6378-82 (1990).
Devlin, James J. et al. “Random Peptide Libraries: A Source of Specific Protein Binding Molecules,”Science249:404-6 (1990).
Dietzel, Christine and Kurjan, Janet “The Yeast SCG1 Gene: A Gα-like Protein Implicated in the a- and α-Factor Response Pathway,”Cell50:1001-10 (1987).
Dmochowska, Aleksandra et al. “YeastKEX1Gene Encodes a Putative Protease with a Carboxypeptidase B-like Function Involved in Killer Toxin and α-Factor Precursor Processing,”Cell50:573-84 (1987).
Dolan, J. W. et al. “Overproduction of the yeast STE12 protein leads to constitutive transcriptional induction,”Genes&Development4(4):492-502 (1990).
Dubois, Patrice M. et al. “Role of the transmembrane and cytoplasmic domains of surface IgM in endocytosis and signal transduction,”Eur. J. Immunol. 22:851-57 (1992).
Erickson, Deborah “Intercepted Messages: New biotechnology drugs target intracellular communication,”Scientific American267(5):122-23 (1992).
Etienne, Gilles et al. “A Screening Method for Antifungal Substances UsingSaccharomyces cerevisiaeStrains Resistant to Polyene Macrolides,”J. of Antibiotics43(2):199-206 (1990).
Fasullo, Michael T. and Davis, Ronald W. “Direction of Chromosome Rearrangements inSaccharomyces cerevisiaeby Use ofhis3 Recombination Substrates,”Molecular and Cellular Biol. 8(10):4370-80 (1988).
Ferrell, James E. Jr. “Tripping the switch fantastic: how a protein kinase cascade can convert graded inputs into switch-like outputs,”Trends in Biochem. Sci. 21(12):460-6 (1996).
Ferrell, James E. Jr. et al. “The Biochemical Basis of an All-or-None Cell Fate Switch inXenopus Oocytes, ” Science280:895-898 (1998).
Fields, Stanley and Song Ok-kyu “A novel genetic system to detect protein-protein interactions,”Nature340:245-46 (1989).
Franke, Arthur E. et al. “Human C5a Anaphylatoxin: Gene Synthesis, Expression, and Recovery of Biologically Active Material fromEscherichia coli,” Methods in Enzymology162:653-68 (1988).
Funaro, Ana et al. “Human CD38 is associated to distinct molecules which mediate transmembrane signaling in different lineages,”Eur. J. Immunol. 23:2407-11 (1993).
Gallego, Carme et al. “Mysristoylation of the Gai2polypeptide, a G prot

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Cell based signal generation does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cell based signal generation, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cell based signal generation will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3597635

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.