Cell-based drug screens for regulators of gene expression

Details Cell-based drug screens for regulators of gene expression Cell-based drug screens for regulators of gene expression

2006-09-12

2006-09-12

Sullivan, Daniel M. (Department: 1636)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

C435S069100, C435S320100, C435S354000

Reexamination Certificate

active

07105313

ABSTRACT:
The invention provides methods and compositions for screening for pharmacological agents which regulate gene expression in mammals. An exemplary assay involves (a) contacting a mammalian cell comprising a knock-in mutant of a targeted native allele encoding a reporter of gene expression, wherein the expression of the reporter is under the control of the gene expression regulatory sequences of the native allele, with a candidate agent under conditions whereby but for the presence of the agent, the reporter is expressed at a first expression level; and, (b) measuring the expression of the reporter to obtain a second expression level, wherein a difference between the first and second expression levels indicates that the candidate agent modulates gene expression.

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