Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2006-10-03
2006-10-03
Ulm, John (Department: 1649)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007200, C435S007210, C436S501000
Reexamination Certificate
active
07115377
ABSTRACT:
Disclosed are compositions and methods for their use, such as in identifying G-protein-coupled receptors, ligands and compounds that modulate the activities of G-protein-coupled receptors. The compositions and methods employ cyclic nucleotide-gated channels and fluorescence dyes in detecting changes of intracellular cAMP levels in response to the stimulation of G-protein-coupled receptors. Activation of the G-protein-coupled receptors can be detected in a variety of assays, including cell-based imaging assays with fluorescence microscopes and high throughput assays with multi-well plates and fluorescence plate readers.
REFERENCES:
patent: 2255548 (2000-06-01), None
patent: WO 98/58074 (1998-12-01), None
McAllister et al., The Journal of Pharmacology and Experimental Therapeutics, 291(2):618-626 (1999).
Wetzel et al., The Journal of Neuroscience, 19(17):7426-7433 (Sep. 1, 1999).
Lee et al., Journal of Neurochemistry, 72(1):58-65 (1999).
Oz et al., Molecular Pharmacology, 54:1106-1112 (1998).
International Searching Authority, PCT/US02/34122.
Thomas C. Rich et al., “In Vivo Assessment of Local Phosphodiesterase Activity Using Tailored Cyclic Nucleotide-Gated Channels as cAMP Sensors”, J. Gen. Physiol., The Rockfeller University Press, Vo.. 118, Jul. 2001, pp. 63-77.
Kent A. Fagan et al., “Adenovirus Encoded Cyclic Nucleotide-Gated Channels: A New Methodology for Monitoring cAMP in Living Cells”, FEBS Letters vol. 500, (2001), pp. 85-90.
Jun Li et al., “Functional Roles of Aromatic Residues in the Ligand-Binding Domain of Cyclic Nucleotide-Gated Channels”, Molecular Pharmacology vol. 55, (1999) pp. 873-882.
Jonathan Bradley et al., “Heteromeric Olfactory Cyclic Nucleotide-Gated Channels: A Subunit That Confers Increased Sensitivity of cAMP”, Proc. Natl. Acad. Sci. USA, vol. 91, Sep. 1994, pp. 8890-8894.
Bradley et al., Functional Expression of the Heteromeric “Olfactory” Cyclic Nucleotide-Gated Channel in the Hippocampus: A Potential Effector of Synaptic Plasticity in Brain Neurons (1997),Jour. of Neurosc.: 1993-2005.
Bradley et al., Receptors that Couple to 2 Classes of G Proteins Increase cAMP and Activate CFTR Expressed inXenopusOocytes (1993)Receptors and Channels1: 233-241.
Dall'Asta et al., Membrane Potential Changes Visualized in Complete Growth Media through Confocal Laser Scanning Microscopy of bis-Oxonol-loaded Cells (1997),Experimental Cell Research: 260-268.
Dhallan et al., Primary structure and functional expression of a cyclic nucleotide-activated channel from olfactory neurons (1990),Nature 347: 184-187.
Dzeja et al., PCa2+permeation in cyclic nucleotide-gated channels (1999),EMBO Jour. 18(1): 131-144.
Fagani et al., Adenovirus-mediated Expression of an Olfactory Cyclic Nucleotide-gated Channel Regulates the Endogenous Ca2+-inhibitable Adenylyl Cyclase in C6-2B Glioma Cells (1999),Jour. Biol. Chem. 274(18): 12445-12453.
Feng et al., Expression of photoreceptor cyclic nucleotide-gated cation channel α) in the liver and skeletal muscle (1996),FEBS Letters395: 77-81.
Frings et al., Profoundly Different Calcium Permeation and Blockage Determine the Specific Function of Distinct Cyclic Nucleotide-Gated Channels (1995)Neutron15: 169-179.
Gavazzo et al., A Point Mutation in the Pore Region Alters Gating, Ca2+Blockage, and Permeation of Olfactory Cyclic Nucleotide-gated Channels (2002),J. Gen Physiol(116): 311-325.
Gerstner et al., Molecular cloning and functional characterization of a new modulatory cyclic nucleotide-gated channel subunit from mouse retina (2002),J. Neurosci. 20(4): 1324-32.
Kramer et al., Modulation of cyclic-nucleotide-gated channels and regulation of vertebrate phototransduction (2001),J. Exper. Biol. (204): 2921-2931.
Laskey et al., Calcium entry-dependent oscillations of cytoplasmic calcium concentration in cultured endothelial cell monolayers (1992),Proc. Natl. Acad. Sci. (89): 1690-1694.
Leinders-Zufall et al.,Imaging Odor-Induced Calcium Transients in Single Olfactory Cilia: Specificity of Activation and Role in Transduction(1998)J. Neurosci. (1998) 18(15): 5630-5339.
Leinders-Zufall et al., Calcium entry through cyclic nucleotide-gated channels in individual cillia of olfactory receptor cells: spatiotemporal dynamics (1997),J. Neurosci. 17(11): 4136-48.
Leinders-Zufall et al., Bl ck of Cyclic Nucleotide-Gated Channels in Salamander Olfactory Receptor Neurons by the Guanylyl Cyclase Inhibitor LY83583 (1995) J. Neurophysiology 74(6): 2759-2762.
Muller, et al., Phosphorylation of mammalian olfactory cyclic nucleotide-gated channels increase ligand sensitivity (1998),J. Neurosci18(1): 164-173.
Nakamura, Cellular and molecular constituents of olfactory sensation in vertebrates (2000), Comp. Biochem. and Physiol. Part A 126(1): 17-32.
Paoletti, et al., C-Linker of cyclic nucleotide-gated channels controls coupling of ligand binding to channel-gating (1999)J. Gen. Physiol. 113(1): 17-34.
Pugh, Transfected Cyclic Nucleotide-gated Channels as Biosensors (2000),J. Gen. Physiol. 116:; 143-145.
Rich, et al., Cyclic Nucleotide-gated Channels Colocalize with Adenylyl Cyclase in Regions of Restricted cAMP Diffusion (2000),J. Gen. Physiol. 116:; 147-161.
Schaad et al., Vasoactive Intestinal Peptide Elevates Pinealocyte Intracellular Calcium Concentrations by Enhancing Influx: Evidence for Involvement of a Cyclic GMP-Dependent Mechanism (1995)Molecular Pharm. 47: 923-933.
Scott, et al. Three Residues Predicted by Molecular Modeling To Interact with the Purine Moiety Alter ligand Binding and Channel Gating in Cyclic Nucleotide-Gated Channels (1998)Biochemistry, 37: 17239-17252.
Shapiro, et al. Structural basis for ligand selectivity of heteromeric olfactory cyclic nucleotide-gated channelS (2000), Biophys. J.78(5): 2307-20.
Terstappen, et al. Pharmacological characterisation of the human small conductance calcium-activated potassium channel hSK3 reveals sensitivity to tricyclic antidepressants and antipsychotic phenothiazines (2001)Neuropharm. 40: 772-783.
Zochowski, et al., Imaging Membrane Potential With Voltage-Sensitive Dyes (2000),Biol. Bull. 198: 1-21.
Wetzel et al., “Phosphorylation of voltage-gated ion channels in rat olfactory receptor neurons,” European J. Neurosci., 14:1056-1064, 2001.
Rich et al., “A uniform extracellular stimulus triggers distinct cAMP signals in different compartments of a simple cell,” Proc. Natl. Acad. Sci. USA, 13049-13054, 2001.
Rich et al., “In vivo assessment of local phosphodiesterase activity using tailored cyclic nucleotide-gated channels as cAMP sensors,” J. Gen. Physiol., 118:63-77, 2001.
Bönigk et al., “The native rat olfactory cyclic nucleotide-gated channel is composed of three distinct subunits,” J. Neurosci., 19:5332-5347, 1999.
Cao Liang
Yao Yong
Atto Bioscience Inc.
Cooley & Godward LLP
Ulm John
LandOfFree
Cell-based assays for G-protein-coupled receptor-mediated... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Cell-based assays for G-protein-coupled receptor-mediated..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cell-based assays for G-protein-coupled receptor-mediated... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3664331