Bleaching and dyeing; fluid treatment and chemical modification – Dye or potential dye composition – additive – treatment,... – Azo – acridone – or quinone dye
Reexamination Certificate
2007-07-03
2007-07-03
Ulm, John (Department: 1649)
Bleaching and dyeing; fluid treatment and chemical modification
Dye or potential dye composition, additive, treatment,...
Azo, acridone, or quinone dye
C008S648000, C430S270190, C435S007100, C435S007200, C435S007210
Reexamination Certificate
active
10978282
ABSTRACT:
Disclosed are novel compositions and methods employing cyclic nucleotide-gated channels and fluorescence dyes and other indicators in detecting changes of intracellular cAMP levels, for instance in response to the stimulation of G-protein-coupled receptors. CNG mutants comprising one or more pore mutations that enhance sensitivity to cAMP and decrease divalent cation-mediated blockage are used in a variety of assays, including detection of the G-protein-coupled receptor activity. Also disclosed are novel dye quencher formulations that are particularly suited for cell-based assays of protein interactions.
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Cao Liang
Llorente Isabel
Lu Jianming
Yao Yong
Atto Bioscience
Cooley Godward Kronish LLP
Ulm John
LandOfFree
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