Cell-based assay for screening cox-2 inhibitors

Details Cell-based assay for screening cox-2 inhibitors Cell-based assay for screening cox-2 inhibitors

2005-08-23

2005-08-23

Rao, Manjunath N. (Department: 1652)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

C435S004000, C435S006120, C435S183000, C435S189000, C435S252300, C435S320100, C435S325000, C536S023200, C536S023700, C514S789000

Reexamination Certificate

active

06933128

ABSTRACT:
A cell-based assay is provided for screening substances for the capacity to inhibit COX-2 activity. The assay utilizes as a screening reagent a cell line which has been engineered to achieve constitutive or inducible expression of COX-2. In one embodiment, the cells express a GTPase-deficient, constitutively-activated mutant form of the α-subunit of protein G12 which induces the production of arachidonic acid and COX-2. Decreased cell proliferation in the presence of test substance indicates that the substance has COX-2 inhibitory activity.

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