Cell- and tissue culture device

Details Cell- and tissue culture device Cell- and tissue culture device

2011-03-01

2011-03-01

Beisner, William H (Department: 1775)

Chemistry: molecular biology and microbiology

Apparatus

Differentiated tissue perfusion or preservation apparatus

C435S173500, C435S297100, C435S297500

Reexamination Certificate

active

07897377

ABSTRACT:
The present invention relates to the field of cell and tissue culture. In particular, the invention provides a novel device for organotypic culture. The device comprises a medium conduit having one open end and one end closed by a porous membrane fused across it; and a frame holding the medium conduit in a substantially vertical orientation; wherein the medium conduit is adapted to permit retention by capillarity of a sufficient volume of liquid culture medium in the medium conduit to contact the surface of the porous membrane and thus supply nutrients to cells that may be grown on the porous membrane. The device may further comprise a cell suspension conduit having one end open and one end closed by the surface of said porous membrane contralateral to the surface of said porous membrane sealed to said medium conduit; wherein said frame holds the medium conduit and the cell suspension conduit in a substantially vertical orientation.

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