Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1995-06-07
2000-06-06
LeGuyader, John L.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 6, 435 9131, 435410, 536 232, 536 245, 800205, C12H 1500, C12Q 168, C12N 1582, A01H 104
Patent
active
060717300
ABSTRACT:
The design of new ribozymes capable of self-catalyzed trans-splicing which are based upon the catalytic core of a Group I intron are described. Using this design, it is possible to construct ribozymes capable of efficiently splicing a new 3' exon sequence into any chosen target RNA sequence in a highly precise manner. A method of cell ablation is also described that provides a toxic product to a host cell in vivo in a targetted, regulated manner utilizing novel trans-splicing ribozymes of the invention. Inactive pro-ribozyme forms are also described.
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Wert, S.E. et al.,
Brand Andrea
Goodman Howard M.
Haseloff James
Perrimon Norbert
LeGuyader John L.
President and Fellows of Harvard College
The General Hospital Corporation
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