Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1995-06-07
1999-01-26
LaGuyader, John L.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 6, 435 9131, 435325, 536 232, 536 245, C12N 1500, C12N 1585, C12Q 168
Patent
active
058637745
ABSTRACT:
The design of new ribozymes capable of self-catalyzed trans-splicing which are based upon the catalytic core of a Group I intron are described. Using this design, it is possible to construct ribozymes capable of efficiently splicing a new 3' exon sequence into any chosen target RNA sequence in a highly precise manner. A method of cell ablation is also described that provides a toxic product to a host cell in vivo in a targetted, regulated manner utilizing novel trans-splicing ribozymes of the invention. Inactive pro-ribozyme forms are also described.
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Brand Andrea
Goodman Howard M.
Haseloff James
Perrimon Norbert
LaGuyader John L.
President and Fellows of Harvard College
The General Hospital Corporation
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