cDNA synthesis improvements

Details cDNA synthesis improvements cDNA synthesis improvements

2006-07-11

2006-07-11

Sisson, Braley L. (Department: 1655)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S005000, C435S006120, C435S015000, C435S091100, C435S091200, C435S068100, C435S070400, C530S350000, C530S351000, C530S324000

Reexamination Certificate

active

07074556

ABSTRACT:
The present invention generally relates to methods of making cDNA molecules and cDNA libraries. The invention also relates to cDNA molecules and cDNA libraries produced according to these methods, as well as to vectors and host cells containing such cDNA molecules and libraries. The invention also relates to kits for making the cDNA molecules and libraries of the invention.

REFERENCES:
patent: 4748233 (1988-05-01), Sloma
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5219989 (1993-06-01), Sonenberg et al.
patent: 5244797 (1993-09-01), Kotewicz et al.
patent: 5338671 (1994-08-01), Scalice et al.
patent: 5374553 (1994-12-01), Gelfand et al.
patent: 5405776 (1995-04-01), Kotewicz et al.
patent: 5587287 (1996-12-01), Scalice et al.
patent: 5595895 (1997-01-01), Miki et al.
patent: 5668005 (1997-09-01), Kotewicz et al.
patent: 5824875 (1998-10-01), Ranu
patent: 5989819 (1999-11-01), Odawara
patent: 6013488 (2000-01-01), Hayashizaki
patent: 6063608 (2000-05-01), Kotewicz et al.
patent: 6096877 (2000-08-01), Vlasuk et al.
patent: 6103473 (2000-08-01), Copeland et al.
patent: 6143528 (2000-11-01), Hayashizaki
patent: 6187544 (2001-02-01), Bergsma et al.
patent: WO 96/10640 (1996-04-01), None
patent: WO 98/08865 (1998-03-01), None
patent: WO 98/44161 (1998-10-01), None
patent: WO 98/45311 (1998-10-01), None
patent: WO 98/47921 (1998-10-01), None
patent: WO 98/51699 (1998-11-01), None
Fuchs, B. et al., “High Temperature cDNA Synthesis by AMV Reverse Transcriptase Improves the Specificity of PCR,”Molecular Biotechnology 12:237-240, Humana Press (1999).
Gerard, G. et al., “Reverse Transcriptase: The Use of Cloned Moloney Murine Leukemia Virus Reverse Transcriptase to Synthesize DNA from RNA,”Molecular Biotechnology 8:61-77, Humana Press (1997).
Nathan, M. et al., “Optimizing Long RT-PCR,”Focus 17:78-80, Life Technologies, Inc. (1995).
Sambrook, J. et al., “Amplification of DNA Generated by Reverse Transcription of mRNA,”Molecular Cloning: A Laboratory Manual. Second Edition, 2:14.20-14.21, Cold Spring Harbor Laboratory Press (1989).
Sambrook, J. et al., “Reverse Transcriptase (RNA-dependent DNA Polymerase),”Molecular Cloning: A Laboratory Manual. Second Edition, 1:5.52-5.55, Cold Spring Harbor Laboratory Press (1989).
Sambrook, J. et al., “Synthesis of the First Strand of cDNA,”Molecular Cloning: A Laboratory Manual.Second Edition, 2:8.11-8.20, Cold Spring Harbor Laboratory Press (1989).
Schwabe, W. et al., “ThermoScript™ RT, a New Avian Reverse Transcriptase for High-Temperature cDNA Synthesis to Improve RT-PCR,”Focus 20:30-33, Life Technologies, Inc. (1998).
Yu, H. and Goodman, M.F., “Comparison of HIV-1 and Avian Myeloblastosis Virus Reverse Transcriptase Fidelity on RNA and DNA Templates,”Journal of Biological Chemistry 267:10888-10896, The American Society for Biochemistry and Molecular Biology, Inc. (1992).
GIBCO BRL Instruction Manual for SUPERSCRIPT™ First-Strand Synthesis System for RT-PCR. Catalog No. 11904-018. Life Technologies, Inc. (2000).
GIBCO BRL Instruction Manual for SUPERSCRIPT™ Preamplification System for First Strand cDNA Synthesis. Catalog No. 18089-011. Life Technologies, Inc. (2000).
Carninci, P. et al., “High-Efficiency Full-Length cDNA Cloning by Biotinylated CAP Trapper,”Genomics 37:327-336, Academic Press, Inc. (1996).
Chenchik, A. et al., “Full-Length cDNA Cloning and Determination of mRNA 5′ and 3′ Ends by Amplification of Adaptor-Ligated cDNA,”BioTechniques 21:526-534, Eaton Publishing Co. (1996).
DeVico, A. et al., “Purification and partial characterization of equine infectious anemia virus reverse transcriptase,”Virology 185:387-394, abstract only, from NCBI PubMed, PMID 1718086 (1991).
“Enzymatic Amplification of RNA by PCR,” in:Current Protocols in Molecular Biology Supplement 17, Ausubel, F., et al., eds., John Wiley and Sons, Inc., New York, New York, pp. 15.4.1-15.4.4 (1992).
Gerard, G., “Reverse Transcriptase: A Historical Perspective,”Focus 20:65-67 (Sep. 1998).
Kaufman, P.B. et al.,Handbook of Molecular and Cellular Methods in Biology and Medicine, CRC Press, Boca Raton, FL (1995), pp. 444-467.
Kotewicz, M.L. et al., “Isolation of cloned Moloney murine leukemia virus reverse trancriptase lacking ribonuclease H activity,”Nucl. Acids Res. 16:265-277 (1988).
Lovett M. et al., “The construction of full length cDNA libraries by conventional methods and a novel double capture technique,”The American Journal of Human Genetics Suppl. to 63:A253, Abstract No. 1456 (Oct., 1998).
Mierendorf, K. and Novy, R., “A High Efficiency cDNA Cloning Vector for Protein Expression, Purification and Substractive Probe Synthesis,”inNovations, Nova Gen, Inc., No. 3, pp. 1-3 (1995).
Mizuno, Y. et al., “Increased specificity of reverse transcription priming by trehalose and oligo-blockers allows high-efficiency window separation of mRNA display,”Nucl. Acids Res. 27:1345-1349 (1999).
Morris, B. et al., “A Novel Strategy for Directional Cloning of Random Primed cDNA,”inNovations, Nova Gen, Inc., No. 3, pp. 1-8 (1995).
Saiki, R.K. et al., “Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase,”Science 239:487-491 (1988).
Soltis, D.A. and Skalka, A.M., “The α and β chains of avian retrovirus reverse transcriptase independently expressed inEscherichia coli: Characterization of enzymatic activities,”Proc. Natl. Acad. Sci. USA 85:3372-3376 (1988).
International Search Report for International Application No. PCT/US00/05138, mailed May 25, 2000.
“Enzymatic Amplification of RNA by PCR,” in:Current Protocols in Molecular Biology Supplement 17, Ausubel, F., et al., eds., John Wiley and Sons, Inc., New York, New York, pp. 15.4.1-15.4.4 (1992).
Zhang, J. and Byrne, C.D., “Differential priming of RNA templates during cDNA synthesis markedly affects both accuracy and reproducibility of quantitative competitive reverse-transcriptase PCR,”Biochem. J.337:231-241, Biochemical Society/Portland Press (Jan. 1999).
Copy of Supplementary Partial European Search Report for European Patent Application No. 00912051, mailed Nov. 19, 2004.
Chou, Q., et al., “Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications,”Nucl. Acids Res.20:1717-1723, Oxford University Press (1992).

Affiliated with

Also associated with

Rating

cDNA synthesis improvements does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with cDNA synthesis improvements, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and cDNA synthesis improvements will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3598339