CDNA of direct-acting fibrinolytic serine protease

Details CDNA of direct-acting fibrinolytic serine protease CDNA of direct-acting fibrinolytic serine protease

1996-10-25

1998-10-13

Grimes, Eric

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

536 232, C12N 964, C12N 1557

Patent

active

058211063

ABSTRACT:
The present invention relates to a novel cDNA of direct-acting fibrinolytic serine protease ("Salmonase") which is prepared from a Korean viper, Salmosa(Agkistrodon halys brevicaudus), and a direct-acting fibrinolytic serine protease deduced therefrom. The cDNA of direct-acting fibrinolytic serine protease contains 699 nucleotides coding for 233 amino acids, and the Salmonase translated therefrom consists of two subunits of 77 and 156 amino acids, respectively. Salmonase cDNA of the invention may be expressed in the proper systems established in the recombinant E. coli, yeast, baculovirus/insect cells and other animal cells, and the recombinant Salmonase prepared therefrom can be practically applied as an active ingredient of thrombolytic and hemostatic agents.

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