cDNA encoding attenuated cell culture adapted hepatitis A virus

Details cDNA encoding attenuated cell culture adapted hepatitis A virus cDNA encoding attenuated cell culture adapted hepatitis A virus

1993-09-13

1995-12-26

Mosher, Mary E.

Chemistry: molecular biology and microbiology

Vector, per se

536 2372, 4352351, C12N 1551

Patent

active

054787467

ABSTRACT:
A full-length cDNA copy of an attenuated, cell culture-adapted hepatitis-A virus genome has been constructed. The HAV cDNA when inserted, without the oligo (dG) oligo (dC) tails, into an RNA transcription vector yielded a plasmid designated pHAV/7. Transfection of monkey kidney cells with pHAV/7 DNA yielded HAV. Transfection with RNA transcripts produced in vitro from pHAV/7 yielded about 10-fold more HAV than transfection with pHAV/7 DNA. HAV thus produced are useful as a vaccine.

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