cDNA cloning methods for the identification of a CD8+ T-lymphocy

Details cDNA cloning methods for the identification of a CD8+ T-lymphocy cDNA cloning methods for the identification of a CD8+ T-lymphocy

1995-06-07

1999-11-30

Scheiner, Laurie

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 724, C12Q 170, G01N 3353

Patent

active

059940549

ABSTRACT:
The present invention relates to a bioactive molecule, herein referred to as the CD8.sup.+ suppressor molecule, that is produced by the CD8.sup.+ subset of human T-lymphocytes and suppresses type-1 human immunodeficiency virus (HIV-1) replication through inhibition of viral transcription. The invention relates to isolation of clonal CD8.sup.+ cells lines that produce the antiviral activity and the development of an assay system for detection of the antiviral activity. The clonal cell lines and the assay system, described herein, may be utilized to purify, characterize and clone the CD8.sup.+ suppressor molecule. The CD8.sup.+ suppressor molecule may have therapeutic applications for treatment of diseases associated with HIV-1 infection.

REFERENCES:
Barker et al., 1995, "Effects of T.sub.H 1 and T.sub.H 2 Cytokines on CD8.sup.+ Cell Response Against Human Immunodeficiency Virus: Implications for Long Term Survival", Proc. Natl. Acad. Sci. USA 92:11135-11139.
Fahey and Schooley, 1992, "Status of Immune-Based Therapies in HIV Infection and AIDS", Clin. Exp. Immunol. 88:1-5.
Fox, 1994, "No Winners Against AIDS", Bio/Technology 12:128.
Kinter et al., 1995, "Interleukin 2 Induces CD8.sup.+ T Cell-Mediated Suppression of Human Immunodeficiency Virus Replication in CD4.sup.+ T Cells and This Effect Overrides Its Ability to Stimulate Virus Expression", Proc. Natl. Acad. Sci. USA 92:10985-10989.
Lacey et al., "Herpesvirus saimiri Transformation of Suppressive CD8.sup.+ T-Lymphocytes from a HIV.sup.+ Asymptomatic Patient", AIDS Res. and Human Retroviruses 10(Suppl. 3):S70.
Levy et al., 1994, "Mechanism of CD8.sup.+ Cell Suppression of HIV Replication", J. Cell. Biochem. Suppl. 18B:111.
Brinchmann et al., 1991, J. Acquired Immune Defic. Syndr. 4:480-488.
Kundu et al., 1992, Viral Immunol. 5:15-25.
Walker et al., 1991, J. Virol. 65:5921-5927.
Powell et al., 1993, Clin. Exp. Immunol. 91:473-481.
Hara et al., 1991, Nuc. Acids Res. 19:7097-7104.
Herfort et al., 1991, BioTech. 11:598-602.

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