Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1992-06-01
1997-10-21
Vogel, Nancy T.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
4353201, 530350, 536 232, 536 235, C12Q 168, C12N 1570, C12N 1511, C07K 1400
Patent
active
056795114
ABSTRACT:
The present invention started when a .lambda.gt11 cDNA library of normal human melanocytes was screened with antibodies directed against purified hamster tyrosinase. Two important cDNA clones were isolated: cDNA .lambda.mel 34 and .lambda.mel 17-1. It is concluded that .lambda.mel 34 contained cDNA encoding human tyrosinase. Moreover, the cDNA gene for human tyrosinase was recovered from the .lambda.mel 34 and can be used for many purposes including the production of pure human tyrosinase. This gene and its promoter are characterized herein. Studies suggested that .lambda.mel 17-1 gene product act on melanin biosynthesis's pathway distal to tyrosinase. Melanocytes preferentially express an mRNA species, Pmel 17, whose protein product cross reacted with anti-tyrosinase antibodies and whose expression correlated with the melanin content. The deduced protein structure has been analyzed and its chromosomal location in mouse and man has been mapped. The human Pmel 17 gene, designated D12S53E, maps to Chromosome 12, region 12pter-q21; and the mouse homologue, designated D12S53Eh, maps to the distal region of mouse Chromosome 10, a region also known to carry the coat color locus si (silver).
REFERENCES:
Houghton et al., J. Exp. Med., vol. 156, pp. 1755-1766 (1982).
Gown et al., Am. J. Pathology, vol. 123, pp. 195-203 (1986).
Deranlowicz et al., Neoplasma, vol. 27 No. 4.
Chang et al., J. Invest. Dermatol. vol. 87 No. 3.
Tomita et al., Diag. Immunol., vol. 4, No. 3.
Vachtenheim et al., J. Invest. Dermatol., vol. 86 No. 3.
Shibahara et al., Nucl. Acids Res., vol. 14, No. 6, pp. 2413-2427 (1986).
Old, Princ. of Gene Manipul., Blackwell Sci. Publ., 3rd ed. pp. 9-10 (1985).
Coleman, D., Effect of Genic Substitution on the Incorporation of Tyrosinase into the Melanin of Mouse Skin, (1962) Arch. Biochem. Biophys, pp. 69, 562-568.
Gluecksohn-Waelsch, S., "Genetic Control of Morphogenetic and Biochemical Differentation: Lethal Albino Deletions in the Mouse", (1979) Cell, 16, 225-227.
Halaban, S. H., "Selective Elimination of Fibroblasts From Cultures of Normal Human Melanocytes", (1984) In Vitro, 20, pp. 447-450.
Halaban, R. et al, "Human Melanocytes Cultured from Nevi and Melanomas", (1986), Jou. of Invest. Dermatoloqy, 87, pp. 95-101.
ROSS, R.A et al, "Expression of a Melanocyte Phenotype in Human Neuroblastoma Cells In Vitro", (1985), Adv. In Neuroblastoma, pp. 249-255.
Chirgwin, J. et al., "Isolation of Biologically Active Ribonucleic Acid from Sources Enriched in Ribonuclease", 1979, Biochem. 18, pp. 5294-5299.
Aviv, H. et al, "Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose" (1972) Proc. Natl. Acad. Sci. USA 69, 1408-1412.
Thomas, P., "Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose", 1980 Proc. Natl. Acad. Sci, USA 77, 5201, 5205.
Gross-Bellard, M. et al., "Isolation of High-Molecular-Weight DNA from Mammalian Cells", (1973) Eur. J.Biochem., 36, pp. 32-38.
Sanger, F. et al, "DNA sequencing with chain-terminating inhibitors", 1977, Proc. Natl. Acad. Sci. USA, 74, pp. 5463-5467.
Biggin, M. et al., "Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination", 1983 Proc. Natl Acad. Sci. USA, 80, pp. 3963-3965.
Goeddel, D. et al, "The structure of eight distinct cloned human leuckoyte interferon cDNAs", 1981, Nature (London) 290, 20-26.
Steiner, D. et al, "Processing Mechanisms in the Biosynthesis of Proteins" 1980, Ann. N.Y. Acad. Sci., 343, pp. 1-16.
Marshall, R., "The Nature and Metabolism of the Carbohydrate-Peptide Linkages of Glycoproteins",1974, Biochem. Soc. Symp. 40, pp. 17-26.
Richardson, J. et al., "Crystal Structure of Bovine Cu, Zn superoxide Dismutase at 3 A Resolution: Chain Tracing and Metal Liquids", 1975, Proc. Natl. Acad. Sci. USA, 72, pp. 1340-1353.
Mason, H., "The Mechanism of The Oxidation of Dihydroxyphenylalanine by Tyrosinase", 1948, J. Bio. Chm. 172, pp. 83-99.
Land, H. et al, "Terminal sequences of eucaryotic mRNA can be cloned with high efficiency", 1981, Nucl. Acid. Res., 9, pp. 2251-2261.
Young, R. et al, "Efficient isolation of genes by using antibody probes", 1983, Proc. Natl. Acad. Sci. 80, pp. 11194-1198.
Blobel, G. et al, "Transfer of Proteins Across Membranes", 1975 J. Cell Biol. 67, pp. 852-862.
Bause, E., "Structural requirement so f N-glycosylation of proteins", 1983 Biochem. J., 209, pp. 331-336.
Nishioka, K., "Particulate Tyrosinase of Human Malignant Melanoma 1978", Eur. J. Biochem., 85, pp. 137-146.
Lerch, J., "An Epr Study of Neurospora Tyrosinase", 1976, FEBS Lett, 69, pp. 161-164.
Messing, J. et al, "A system for shotgun DNA sequencing" 1981, Nucleic Acids Res. 9, pp. 309-322.
Beermann, F. et al., Rescue of albino phenotype by introduction of a functional tyrosinase gene into mice, 1990, EMBO J., 9:2819-2826.
Charthew, R. et al, An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter, 1985, Cell., 43: 439-448.
Green, M.C., (1961) Journal of Heredity, 52, 73-75.
Kidson, S.H. et al, 1981, JournaL of Exp. Zoology 215, 91-97.
Southern, E.,-1975-J. Mol. Biol. 87, 503-517.
Biossy, R. et al, 1986, J. of Investigative Dermatology, 88, 292-300.
Witkop, C. Jr., 1984, The Clinics in Dermatology, vol.2, 70-134.
Halaban, R. et al., 1983, J. Cell Biol., 97, 480-488.
Mehra, V. et al., 1986, Proc. Natl. Acad. Sci. USA 83, 7013-7017.
Shibahara, S. 1987, Advance in Pigment Cell Research, pp. 263-271.
Kwon, et al, Isolation and initial characterization of multiple species of T-lymphocyte subset cDNA clones, Pro. Natl. Acad. Sci USA, Vo. 84. pp. 2896-2900, May 1987.
Yamamoto et al, Cloning and sequencing of mouse tyrosianse cDNA, Jpn, J. Genet. (1987) 62, pp. 271-274.
Shibahara, S. et al., Cloning and expression of cDNA encoding mouse tyrosinyase, IRL Press Limited, Oxford, GB., pp. 2413-2427.
Chodosh, L. et al., "A single polypeptide possess the binding and transcription activities of the adenovirus major late transcription factor" 1986, Mol. Cell. Biol., 6:4723-4733.
Dignam, J. et al, "Accurate transcription initiation by RNA Polymerase II in a soluble extract from isolated mammalian nuclei", 183, Nucleic. Acids. Res., 11: 1475-1489.
Emerson, B. et al., "Interaction of specific nuclear factors with the nuclease-hypersensitive region of the chicken adult .beta.-globin gene", 1985, Cell. 41:21-30.
Kadonaga, J. et al., "Affinity purification of sequence-specific DNA binding proteins", 1986 Proc. Natl. Acad. Sci. USA, 83:5889-5893.
Kluppel, M. et al., "The mouse tyrosinase promoter is sufficient for expression in melanocytes and in the pigmented epithelium of the retina", 1991, Proc. Natl. Acad. Sci. USA., 88:3777-3781.
Kwon, B. et al., "Sequence analysis mouse tyrosinase cDNA and the effects of melanotropin on its gene expression", 1988, Biochem. Biophys. Res. Comm., 153:1301-1309.
Kwon, B. et al., "Isolation, chromosomal mapping and expression of the mouse tyrosinase gene", 1989 J. Invest. Dermotol., 93:589-594.
Muller, G. et al, "Functional analysis of alternatively spliced tyrosinase gen transcripts", 1988 EMBO J., 7:2723-2730.
Yokoyama, T. et al, "Conserved cysteine to serine, mutation in tyrosinase is responsible for the classical albino mutation in laboratory mice", 1990, Nucleic. Acids. Res., 18:7293-7298.
Barton, D. et al., "Human tyrosinase gone mapped to chromosome 11(q14-q21) defines second region of homology with mouse chromosome 7", 1988, Genomics. 3:17-24.
Bouchard, B. et al, "Induction of pigmentation in mouse fibroblasts by expression of human tyrosinase cDNA", 1989 J. Exp. Med. 169:2029-2042.
Chintamaneni, C. et al., "A single base insertion in the putative transmembrane domain of the tyrosinase gene as cause for tyrosinase-negative oculocutaneous albinism", 1991, Proc. Natl. Sci. USA, 88:5272-5276.
Giebel, L. et al., "Organization and nucleotide sequence of the human tyrosinase gene and a truncated tyrosinase-related segment", 1919, Genomics., 9:435-445.
Halaban, R. et al., "The dual effect of melanocyte-stimulating hormone (MSH) on the growth of cultured mouse melanoma cells",
Donald Guthrie Foundation for Medical Research, Inc.
Indiana University Foudation
Vogel Nancy T.
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