CDI1 polypeptides

Details CDI1 polypeptides CDI1 polypeptides

1995-06-05

1998-07-28

Sisson, Bradley L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4352523, 4353201, 530324, C12P 2106, C12N 1500, A61K 3800

Patent

active

057861692

ABSTRACT:
Disclosed is a method for determining whether a first protein is capable of physically interacting with a second protein. The method involves: (a) providing a host cell which contains (i) a reporter gene operably linked to a protein binding site; (ii) a first fusion gene which expresses a first fusion protein, the first fusion protein including the first protein covalently bonded to a binding moiety which is capable of specifically binding to the protein binding site; and (iii) a second fusion gene which expresses a second fusion protein, the second fusion protein including the second protein covalently bonded to a weak gene activating moiety; and (b) measuring expression of the reporter gene as a measure of an interaction between the first and the second proteins. Such a determination facilitates the isolation of the gene encoding the interacting protein. Also disclosed herein is recombinant Cdi1 polypeptide, nucleic acid encoding the Cdi1 polypeptide, and uses thereof.

REFERENCES:
patent: 5283173 (1994-02-01), Fields et al.
Dalton and Treisman, Cell 68:597-612, 1992.
Touchette, The Journal of NIH Research 3:44-46, 1991.
Chien et al., Proc. Natl. Acad. Sci. USA 88:9578-9582, 1991.
Fields and Song, Nature 340:245-246, 1989.
Fearon et al., Proc. Natl. Acad. Sci. USA 89:7958-7962 (1992).
Gyuris et al., Cell 75:791-803 (1993).
Hannon et al., Proc. Natl. Acad. Sci USA 91:1731-1735 (1994).
Yang et al., Science 257:680-682 (1992).
Meyerson et al., EMBO J. 11:2909-2917, 1992.
Koff et al., Cell 66:1217-1228, 1991.
Tsai et al., Nature 353:174-177, 1991.
Xiong et al., Cell 65:691-699, 1991.
Draetta, Trends in Biochem. Sci. 15:378-382, 1990.
Richardson et al., Genes & Dev. 4:1332-1344, 1990.
Wittenberg et al., Cell 62:225-237, 1990.
Richardson et al., Cell 59:1127-1133, 1989.
Wittenberg et al., Moll. Cell. Biol. 9:4064-4068, 1989.
Pines et al., Cell 59:833-846, 1989.
Hadwiger et al., Proc. Natl. Acad. Sci. USA 86:6255-6259, 1989.
Wittenberg et al., Cell 54:1061-1072, 1988.
Gill et al., Nature 334:721-724, 1988.
Brent et al., Nature 312:612-615, 1984.
Brent et al., Cell 43:729-736, 1985.
Broach et al., Gene 8:121-133, 1979.
Celenza et al., Science 233:1175-1180, 1986.
Celenza et al., Mol. Cell. Biol. 9:5045-5054, 1989.
Celenza et al., Mol. Cell. Biol. 9:5034-5044, 1989.
Curran et al., Cell 55:395-397, 1988.
Dang et al., Mol. Cell. Biol. 11:954-962, 1991.
Furey et al., Science 231:704-707, 1986.
Gill et al., Cell 51:121-126, 1987.
Goff et al., Genes & Dev. 6:864-875, 1992.
Hardy et al., Genes & Dev. 6:801-814, 1992.
Hope et al., Cell 46:885-894, 1986.
Hope et al. Nature 333:635-640, 1988.
Hu et al., Science 250:1400-1403, 1990.
Johnston, Microbiological Reviews 51: 458-476, 1987.
Keegan et al., Science 231:699-704, 1986.
Kumar et al., Cell 51:941-951, 1987.
Laughon et al., Mol. Cell. Biol. 4:260-267 1984.
Ma et al., Cell 55:443-446, 1988.
Ma et al., Cell 48:847-853, 1987.
Martin et al., Mol. Cell. Biol. 10:1908-1914, 1990.
McKnight et al., Proc. Natl. Acad. Sci. USA 84:7061-7065, 1987.
Silver et al., Proc. Natl. Acad. Sci. USA 81:5951-5955, 1984.
Thukral et al., Mol. Cell. Biol. 9:2360-2369, 1988.
Wittekind et al., Mol. Cell. Biol. 8:3997-4008, 1988.
Ma et al., Cell 51:113-119, 1987.
Berger et al., Cell 70:251-265, 1992.

Affiliated with

Also associated with

Rating

CDI1 polypeptides does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with CDI1 polypeptides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and CDI1 polypeptides will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-22365