CD8alpha mutants

Drug – bio-affecting and body treating compositions – Nonspecific immunoeffector – per se ; or nonspecific...

Reexamination Certificate

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Details

C514S002600, C514S885000, C530S350000, C530S868000

Reexamination Certificate

active

07425339

ABSTRACT:
The present invention provides modified CD8 molecules whose binding to MHC is enhanced compared to wild-type CD8, wherein Ser53of at least one CD8α chain thereof is mutated to another amino acid. It also provides nucleic acids encoding such molecules and methods of using such molecules and nucleic acids in immunosuppressive therapy, in particular as inhibitors of cytotoxic T cell responses.

REFERENCES:
patent: WO 99 21576 (1999-05-01), None
Gao, et al, “Molecular Interactions of Coreceptor CD8 and MHC Class I: the molecular basis for functional coordination with the T-cell receptor”, Immunology Today, Dec. 1, 2000, pp. 630-636, vol. 21, No. 12, Elsevier Publications, GB.
Sewell et al., “Antagonism of Cytotoxic—T-lymphocyte Activation by Soluble CD8,” Nature Medicine, Apr. 1999, pp. 399-404, vol. 5, No. 4, Nature Medicine, USA.
Kern, et al., “Structural Basis of CD8 Coreceptor Function Revealed by Crystallographic Analysis of a Murine CD8 Alphaalpha Ectodomain Fragment in Complex with H-2Kb,” Immunity, Oct. 1998, pp. 519-530, vol. 9, No. 4, Cell Press, USA.
Li et al., “Identification of the CD8 DE Loop as a Surface Functional Epitope. Implications for Major Histocompatibility Complex Class I Binding and CD8 Inhibitor Design,” The Journal of Biological Chemistry, Jun. 26, 1998, pp. 16442-16445, vol. 273, No. 26, Amer. Soc. For Biochem. And Mol. Bio., Inc., USA.
Giblin et al., “A secreted form of the human lymphocyte cell surface molecule CD8 arises from alternative splicing,”Proc. Natl. Acad. Sci. USA 86, 998-1002, Feb. 1989.

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