CD8.sup.+ cell antiviral factor

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 724, 435 734, 435 5, 435 792, 530350, G01N 3353, G01N 33569, G01N 33542, C07K 100

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active

057078149

ABSTRACT:
A protein secreted by peripheral blood mononuclear cells and specifically from CD8.sup.+ lymphocytes is purified and characterized here and designated CD8.sup.+ cell antiviral factor (CAF). The CAF is lipid free and has been found to inhibit the replication of retroviruses and in particular to inhibit the replication of HIV-1, HIV-2, and SIV. CAF can be used to inhibit viral RNA transcription which inhibits vital replication. Accordingly, CAF can be used to treat patients infected with retroviruses. The CAF can also generate antibodies which can be used to create assay devices for detecting CAF in a body fluid and on the surface of CD8.sup.+ cells to determine the condition of an HIV infected individual.

REFERENCES:
Brinchman, et al, 1990, "CD8.sup.+ TCells Inhibit HIV Replication . . . " J. Immunol. 144:2961-2966.
Walker, et al, 1986, "CD8.sup.+ Lymphocytes Can Control HIV Infection . . . " Science 234:1563-1566.
Walker, et al, 1989, "A Diffusable Lymphokine Produced by . . . " Immunology 66:628-630.

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