CCD imager for confocal scanning microscopy

Facsimile and static presentation processing – Facsimile – Picture signal generator

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358483, H04N 314

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active

059009497

ABSTRACT:
A CCD array imager adapted for use as a spatial noise discriminator in confocal scanning microscopy comprises a parallel (vertical) register with a horizontal unmasked imaging row of pixels in tandem with a masked storage array, a serial (horizontal) register for readout, and a means for synchronizing image acquisition in the unmasked row with vertical charge shifting in the storage array such that images are mapped to on-diagonal pixels of the storage array whereas noise is mapped to off-diagonal pixels. A method for using the present invention as a spatial noise discriminator for fluorescence microscopy includes acquiring a fluorescence image in a pixel of the unmasked row of the parallel register, vertically shifting the charges accumulated in the row into the storage array, moving the next fluorescent image horizontally to the next position in the unmasked row, repeating the process to map image information to the on-diagonal pixels of the storage array while segregating noise in the off-diagonal pixels and selectively retrieving image information.

REFERENCES:
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Methods in Cell Biology, Chapter 13, pp. 207-237, and Chapter 16, pp. 291-313,vol. 29, Part A, Eds. Y-L. Wang & D.L. Taylor, 1989.
Electronic Light Microscopy, Chapter 10, pp. 231-246, and Chapter 12, pp. 273-287, Ed. D. Shotton, A. John Wiley, 1993.
G.J. Brakenhoff and K. Visscher, Novel Confocal Imaging and Visualization Techniques, Trans. R. Microsc. Soc., vol. 1, pp. 247-250, 1990.
G.J. Brakenhoff and K. Visscher, Confocal Imaging with Bilateral Scanning and Array Detectors, Scanning, vol. 13, Suppl. 1, pp. 65-66, 1991.

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