CCD imager for confocal scanning microscopy

Image analysis – Image sensing

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358458, H04N 314

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active

060849916

ABSTRACT:
A CCD array imager adapted for use as a spatial noise discriminator in confocal scanning microscopy comprises a parallel (vertical) register with a horizontal unmasked imaging row of pixels in tandem with a masked storage array, a serial (horizontal) register for readout, and a means for synchronizing image acquisition in the unmasked row with vertical charge shifting in the storage array such that images are mapped to on-diagonal pixels of the storage array whereas noise is mapped to off-diagonal pixels. A method for using the present invention as a spatial noise discriminator for fluorescence microscopy includes acquiring a fluorescence image in a pixel of the unmasked row of the parallel register, vertically shifting the charges accumulated in the row into the storage array, moving the next fluorescent image horizontally to the next position in the unmasked row, repeating the process to map image information to the on-diagonal pixels of the storage array while segregating noise in the off-diagonal pixels and selectively retrieving image information.

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