Cationic lipid: DNA complexes for gene targeting

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Carbohydrate doai

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435 691, 435 911, 4351721, 4351723, 4353201, 435375, 435 701, 435 6, 424450, A61K 4800, C12N 1564, C12N 1585, C12N 1588

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058308784

ABSTRACT:
This invention herein describes pharmaceutical compositions and methods for targeted delivery of functional genes into cells and tissues in vivo. The invention discloses DNA:lipid complexes, methods of making such complexes and methods of using such complexes for facilitating the targeted delivery and entry of recombinant expression constructs into cells and tissues in vivo, and particularly delivery of such recombinant expression constructs by intravenous, intraperitoneal or direct injection.

REFERENCES:
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Sambrook et al., 1990, Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Press: New York).
Thomas & Capecchi, 1987, "Site-Directed Mutagenesis by Gene Targeting in Mouse Embryo-Derived Stem Cells," Cell 51:503-512.
Bertling, 1987, "Transfection of a DNA/Protein Complex into Nuclei of Mammalian Cells Using Polyoma Capsids and Electroporation," Bioscience Reports 7:107-112.
Smithies et al., 1985, "Insertion of DNA sequences into the human chromosomal .beta.=globin locus by homologous recombination," Nature 317:230-234.
Innis et al., 1990, PCR Protocols: A Guide to Methods and Applications, Academic Press, New York.
Solodin et al., 1995, "A novel series of amphiphilic imidazolinium compounds for in-vitro and in-vivo gene delivery," Biochemistry 34, No. 41, 17 Oct. 1995.
Poste et al., 1974, "Lipid vesicles as carriers for introducing biological active materials into cells," Methods Cell. Biol. 14:33-71.

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