Catalyzed reporter deposition

Details Catalyzed reporter deposition Catalyzed reporter deposition

1996-05-20

1998-03-24

Adams, Donald E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 71, 435 771, 435 772, 435 79, 435 791, 435 15, 435 18, 435 21, 435 25, 435 28, 436501, 436518, G01N 3353

Patent

active

057311587

ABSTRACT:
The present invention concerns a method to catalyze reporter deposition to improve detection or quantitation of an analyte in a sample by amplifying the detector signal which comprises reacting an analyte dependent enzyme activation system with a conjugate consisting of a detectably labeled substrate specific for the enzyme system, said conjugate reacts with the analyte dependent enzyme activation system to form an activated conjugate which deposits substantially wherever receptor for the activated conjugate is immobilized, said receptor not being reactive with the analyte dependent enzyme activation system.
In another embodiment the invention concerns an assay for detecting or quantitating the presence or absence of an analyte in a sample using catalyzed reporter deposition to amplify the reporter signal.

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