Chemistry: electrical and wave energy – Apparatus – Electrolytic
Patent
1992-05-15
1993-07-13
Niebling, John
Chemistry: electrical and wave energy
Apparatus
Electrolytic
204416, 204418, 435288, 435291, 435817, G01N 2726
Patent
active
052270424
ABSTRACT:
An enzyme electrode is prepared with a composite coating on an electrical conductor. The composite coating is formed from a casting solution of a perfluorosulfonic acid polymer, an enzyme, and a carbon supported catalyst. The solution may be cast directly on the conductor surface or may be formed as a membrane and applied to the surface. The perfluorosulfonic acid ionomer formed from the casting solution provides an insoluble biocompatible protective matrix for the enzyme and acts to retain the enzyme for long term availability in the electrode structure. The carbon supported catalyst provides catalytic sites throughout the layer for the oxidation of hydrogen peroxide from the enzyme reactions. The carbon support then provides a conductive path for establishing an electrical signal to the electrical conductor. In one embodiment, the electrical conductor is a carbon cloth that permits oxygen or other gas to be introduced to the perfluorosulfonic polymer to promote the enzyme reaction independent of oxygen in the solution being tested.
REFERENCES:
patent: 4970145 (1990-11-01), Bennetto et al.
patent: 5082550 (1992-01-01), Risphon et al.
patent: 5160418 (1992-11-01), Mullen
H. P. Bennetto et al., "A Glucose Oxidase Electrode for Amperometric Determination of Glucose," International Industrial Biotechnology #2, pp. 5-10 (Mar./Apr. 1988).
Gottesfeld Shimshon
Rishpon Judith
Wilson Mahlon S.
Zawodzinski Thomas A.
Bell Bruce F.
Gaetjens Paul D.
Moser William R.
Niebling John
The United States of America as represented by the United States
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