Catalytic DNA

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435199, 536 245, C12P 1934, C12N 1511

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active

058612882

ABSTRACT:
Nucleic acid able to cause specific cleavage of a bond between two ribonucleotides in an RNA-containing molecule. The RNA-containing molecule has the structure:

REFERENCES:
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Perreault et al., "Mixed Deoxyribo- and Ribo-Oligonucleotides with Catalytic Activity," Nature 344:565-567 (1990).
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Shimayama et al., "Nuclease-resistant chimeric ribozymes containing deoxyribonucleotides and phosphorthioate linkages," Nucleic Acids Research 21:2605-2611 (1993).
Jeffries and Symons, "A Catalytic 13-mer Ribozyme," 17 Nucleic Acids Research 1371, 1989.
Yang et al., "Mixed DNA/RNA Polymers Are Cleaved by the Hammerhead Ribozyme", 29 Biochemistry 11156, 1990.
Perreault et al., "Relationship between 2'-Hydroxyls and Magnesium Binding in the Hammerhead RNA Domain: A Model for Ribozyme Catalysis", 30 Biochemistry 4020, 1991.
Yang et al., "Minimum ribonucleotide Requirement for Catalysis by the RNA Hammerhead Domain", 31 Biochemistry 5005, 1992.
Usman and Cedergren, "Expoloiting the chemical synthesis of RNA", 17 Trends in Biochem Sci. 334, 1992.
Rossi et al., "Ribozymes as Anti-HIV-1 Therapeutic Agents: Principles, Applications, and Problems," 8 Aids Research and Human Retroviruses 183, 1992.
Hampel and Tritz, "RNA Catalytic Properties of the Minimum (-)sTRSV Sequence," 28 Biochemistry 4929, 1989.
Hampel et al., "`Hairpin` Catalytic RNA Model: Evidence for Helices and Sequence Requirement for Substrate RNA," 18 Nucleic Acids Research 299, 1990.
Perrotta and Been, "cleavage of Oligoribonucleotides by a Ribozyme Derived from the Hepatitis .delta. Virus RNA Sequence," 31 Biochemistry 16, 1992.

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