Catalyst mediated competitive protein binding assay

Details Catalyst mediated competitive protein binding assay Catalyst mediated competitive protein binding assay

1977-07-14

1979-07-10

Marantz, Sidney

Chemistry: physical processes

Physical processes

Crystallization

23230R, 424 12, G01N 3316, G01N 3110, G01N 2122

Patent

active

041606450

ABSTRACT:
Method and compositions are provided for competitive protein binding assays wherein a non-enzymic catalytically mediated reaction is sterically inhibited by receptors binding to a ligand to which the reaction intermediary is bound. The method employs first and second reactants, which only react with each other either very slowly or not at all. The reaction intermediary or non-enzymic catalyst provides for a two step reaction having as its final result the transformation of the first and second reactants. By conjugating the reaction intermediary to a ligand, whereby the presence of receptor bound to the ligand inhibits the approach of one or both of the reactants, ligand and antiligand can be determined. Alternatively, anticatalyst can be employed which is inhibited from binding to the catalyst when antiligand is bound to ligand. The binding of anticatalyst to catalyst inhibits the catalytically mediated reaction. By comparing the rate of reaction with an unknown amount of analyte present to rates with known amounts of analyte, the concentration of the analyte in an unknown may be determined.

REFERENCES:
patent: 3899298 (1975-08-01), Szezesniak
patent: 3935074 (1976-01-01), Rubenstein
patent: 3966556 (1976-06-01), Rubenstein
patent: 3996345 (1976-12-01), Ullman

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