Cast-to-shape electrokinetic trapping medium

Details Cast-to-shape electrokinetic trapping medium Cast-to-shape electrokinetic trapping medium

2006-05-30

2006-05-30

Therkorn, Ernest G. (Department: 1723)

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

C210S656000, C210S198200, C210S502100, C204S455000, C521S063000

Reexamination Certificate

active

07052608

ABSTRACT:
A three-dimensional microporous polymer network material, or monolith, cast-to-shape in a microchannel. The polymer monolith, produced by a phase separation process, is capable of trapping and retaining charged protein species from a mixture of charged and uncharged species under the influence of an applied electric field. The retained charged protein species are released from the porous polymer monolith by a pressure driven flow in the substantial absence of the electric field. The pressure driven flow is independent of direction and thus neither means to reverse fluid flow nor a multi-directional flow field is required, a single flow through the porous polymer monolith can be employed, in contrast to prior art systems. The monolithic polymer material produced by the invention can function as a chromatographic medium. Moreover, by virtue of its ability to retain charged protein species and quantitatively release the retained species the porous polymer monolith can serve as a means for concentrating charged protein species from, for example, a dilute solution.

REFERENCES:
patent: 6136187 (2000-10-01), Zare et al.
patent: 6472443 (2002-10-01), Shepodd
patent: 6616825 (2003-09-01), Frechet et al.
Quirino, J. P. et al., “Strategy for on-line Preconcentration in Chromatographic Separations”, Anal. Chem., 73, 5539, Nov. 15, 2001.
Quirino, J. P. et al., “On-line Preconcentration in Capillary Electrochromatography Using a Porous Monolith together with Solvent Gradient and Sample Stacking”, Anal. Chem., 73, 5557, Nov. 15, 2001.
Dulay, M. T. et al., “Photopolymerization Sol-gel Monoliths for Capillary Electrochromatography”, Anal. Chem., 73, 3921, Aug. 15, 2001.

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