Cascade immunoassay by multiple binding reactions

Details Cascade immunoassay by multiple binding reactions Cascade immunoassay by multiple binding reactions

1987-05-26

1990-02-27

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 18, 435 19, 435 21, 435 23, 435175, 435181, 435267, 435810, 435814, 436505, 436518, 436537, 436538, 436540, 436543, 436544, 436547, 436800, 436808, 436817, 436829, G01N 3353, G01N 33543, G01N 33537, G01N 33532

Patent

active

049045830

ABSTRACT:
A method for enzyme immunoassay includes contacting under binding conditions a liquid suspected of containing an analyte, an antianalyte affixed to a solid support and a tracer having an enzyme conjugated thereto. A bound fraction is separated from the liquid and incubated in a second liquid with a masked ligand. The masked ligand is converted by the enzyme on the bound fraction to give free lignad which binds to an antiligand. A signal system, such as a signal enzyme and substrate therefor, or a label-loaded vesicle and vesicle lysing agent, is added to generate a signal used to detect or measure the analyte in the liquid. The invention includes a kit of materials useful in performing the assay of the invention.

REFERENCES:
patent: 4463090 (1984-07-01), Harris
patent: 4483929 (1984-11-01), Szoka
patent: 4543325 (1985-09-01), Albert et al.
patent: 4595655 (1986-06-01), Self
patent: 4649105 (1987-03-01), Kasahara et al.
patent: 4703017 (1987-10-01), Campbell et al.
patent: 4746631 (1988-05-01), Clogett

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