Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1981-09-30
1984-07-31
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 13, 435 23, 435188, 435810, 435 24, 436829, G01N 3354, C12Q 156, C12Q 138, C12N 996
Patent
active
044630905
ABSTRACT:
The invention provides enzyme immunoassays whose sensitivity is increased by cascade amplification. The coupled ligand (enzyme or an activator) catalytically activates a second enzyme which acts on a substrate or can act on a third enzyme to produce a cascade. Alternatively, a proenzyme is coupled to the ligand and converted by an activator to an enzyme which is itself an activator of a second proenzyme in a cascade.
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