Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is inorganic
Patent
1996-10-15
1998-02-03
Wolski, Susan
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is inorganic
128770, 422 55, 422 58, 422 8205, 435 792, 435 794, 435970, 435975, 436 63, 436164, 436800, 436805, 436808, 436824, 604272, 604327, 604411, 604416, 606167, 606181, 606185, G01N 33553
Patent
active
057143900
ABSTRACT:
The invention is a device and a method for collecting samples mixing the samples with test reagents, and acting as a container in which the mixture can be incubated and the test reaction viewed by microscope or imaging device. This device enables an entire test to be performed in one simple step without complicated handling procedures. The device consists of a cartridge with a well with micro-lances imbedded in the bottom of the well and an overlying micro-baggy containing a mixture of reagents. There are two reagents present in the micro-baggy: the first consisting of antibodies coupled to paramagnetic microspheres and the second consisting of antibodies coupled with a fluorochrome. A test subject presses down onto the micro-baggy and at the same time punctures his/her finger or thumb on the micro-lances. Once the finger has been lanced, breaking the micro-baggy, the reagents mix with the test subject's blood. The well is then covered by a clear mylar strip. After incubation the cartridge is moved to a viewing platform. A magnetic gradient is applied to the side of the well causing the magnetic separation of antibodies bound to infected peripheral blood lymphocytes from uninfected peripheral blood lymphocytes. A focused light source is applied to the well, causing antibodies bound to infected peripheral blood lymphocytes if any are present, to glow at the specific emmision frequency determined by the specific fluorochome. The test subject can be identified by a bar code strip attached to the cartridge.
REFERENCES:
patent: 3203540 (1965-08-01), Kalt
patent: 3272319 (1966-09-01), Brewer
patent: 4122947 (1978-10-01), Falla
patent: 4329317 (1982-05-01), Detweiler et al.
patent: 4365970 (1982-12-01), Lawrence
patent: 4382062 (1983-05-01), Kohl
patent: 4777964 (1988-10-01), Briggs
patent: 4935147 (1990-06-01), Ullman
patent: 5054499 (1991-10-01), Swierczek
patent: 5186827 (1993-02-01), Liberti
patent: 5201324 (1993-04-01), Swierczek
patent: 5231993 (1993-08-01), Haber et al.
patent: 5238810 (1993-08-01), Fujiwara
Hallowitz Robert A.
King Chester F.
Bio-Tech Imaging, Inc.
Wolski Susan
LandOfFree
Cartridge test system for the collection and testing of blood in does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Cartridge test system for the collection and testing of blood in, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cartridge test system for the collection and testing of blood in will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-661727