Cartilage composites and methods of use

Details Cartilage composites and methods of use Cartilage composites and methods of use

2006-08-08

2006-08-08

Witz, Jean C. (Department: 1651)

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Animal or plant cell

Reexamination Certificate

active

07087227

ABSTRACT:
Disclosed are neocartilage compositions characterized by having multiple layers of cells, said cells being surrounded by a substantially continuous insoluble glycosaminoglycan and collagen-enriched hyaline extra-cellular matrix, and which neocartilage phospholipids are advantageously enriched in anti-inflammatory n-9 fatty acids, particularly 20:3 n-9 eicosatrienoic or Mead acid.Also disclosed are methods of growing neocartilage in substantially serum-free growth media and methods of producing a conditioned growth media containing compounds effective to enhance neocartilage formation.The neocartilage compositions are useful as implants and as replacement tissue for damaged or defective cartilage and as a model system for studying articular cartilage disease and response to natural and synthetic compounds.

REFERENCES:
patent: 4642120 (1987-02-01), Nevo et al.
patent: 4681763 (1987-07-01), Nathanson et al.
patent: 4846835 (1989-07-01), Grande
patent: 4877864 (1989-10-01), Wang et al.
patent: 5041138 (1991-08-01), Vacanti et al.
patent: 5326357 (1994-07-01), Kandel
patent: 5368051 (1994-11-01), Dunn et al.
patent: 5585237 (1996-12-01), Oppermann et al.
patent: 5658882 (1997-08-01), Celeste et al.
patent: 5688678 (1997-11-01), Hewick et al.
patent: 5709854 (1998-01-01), Griffith-Cma et al.
patent: 5723331 (1998-03-01), Tubo et al.
patent: 5736372 (1998-04-01), Vacanti et al.
patent: 5770193 (1998-06-01), Vacanti et al.
patent: 5786217 (1998-07-01), Tubo et al.
patent: 5902741 (1999-05-01), Purchio et al.
patent: 6110482 (2000-08-01), Khouri et al.
patent: 0739631 (1996-10-01), None
patent: WO 94/09118 (1994-04-01), None
patent: WO 98/04681 (1998-02-01), None
Chang et al., “Cartilage-Derived Morphogenetic Proteins,” J. Biol. Chem., 1994, pp. 28227-28234, vol. 269.
Xu et al., “Dietary Lipids Modify the Fatty Acid Composition of Cartilage, Isolated Chondrocytes and Matrix Vesicles,” LIPIDS, 1994, pp. 619-625, vol. 29.
Adkisson et al., FASEB J., 1991, pp. 344-353, vol. 5.
Brittberg et al., “Treatment of Deep Cartilage Defects in the Knee with Autologous Chondroxyte Transplantantion,” N. Eng. J. Med., 1994, pp. 889-895, vol. 331.
Cleland et al., “Inhibition of Human Neutrophil Leukotriene B4Synthesis in Essential Fatty Acid Deficiency: Role of Leukotriene A Hydrolase,” Lipids, 1994, pp. 151-155, vol. 29.
Elves, “A Study of the Transplantation Antigens on Chondrocytes from Articular Cartilage,” J. Bone Joint Surg., 1974, pp. 178-185, vol. 56B.
Fedewa et al., “Chondrocytes in Culture Produce a Mechanically Functional Tissue,” J. Orthopaedic Research, 1998, pp. 227-236, vol. 16.
Gertzbein et al., “The Stimulation of Lymphocytes by Chondroxytes in Mixed Cultures,” Clin. Exp. Immunol., 1976, pp. 102-109, vol. 24.
Hale et al., “Development of a New Serum-Free Medium, USC-HCI, for Growth and Normal Phenotype in Postembryonic Chicken Growth Plate Chondrocytes,” In Vitro Cellular & Developmental Biology, 1986, pp. 597-603, vol. 22.
Jahn et al., “Changes in Cell Surface Antigen Expression on Human Articular Chondroxytes Induced by Gamma-Interferon,” Arthritis & Rheumatism, 1987, pp. 64-74, vol. 30.
Jennings et al., “Clonal Growth of Primary Cultueres of Rabbit Ear Chondrocytes in a Lipid-Supplemental Defined Medium,” Experimental Cell Research, 1983, pp. 415-423, vol. 145.
Jobanputra et al., “Cellular Responses to Human Chondrocytes: Absence of Allogeneic Responses in the Presence of HLA-DR and ICAM-1,” Clin. Exp. Immunol., 1992, pp. 336-344, vol. 90.
Jurgensen et al., “A New Biological Glue for Cartilage-Cartilage Interfaces: Tissue Transglutaminase,” J. Bone and Joint Surg., 1997, pp. 185-193, vol. 79A.
Lee et al., “Conversion to an Elastogenic Phenotype by Fetal Hyaline Chondroxytes is Accompanied by Altered Expression of Elastin-Related Macromolecules,” Dev. Biol., 1994, pp. 241-252, vol. 163.
Osman et al., “Combined Transgenic Expression of α1 2-Fucosyltransferase Leads to Optimal Reduction in the Major Xenoepitope Galα(1,3)Gal,” Proc. Natl. Acad. Sci. USA, 1997, pp. 14677-14682, vol. 94.
Reginato et al., “Formation of Nodular Structures Resembling Mature Articular Cartilage in Long-Term Primary Cultures of Human Fetal Epiphyseal Chondrocytes on a Hydrogel Substrate,” Arthritis & Rheumatism, 1994, pp. 1338-1349, vol. 37.
Roughley et al., Biochem. J., 1993, pp. 421-426, vol. 295.
Sandrin et al., “Enzymatic Remodelling of the Carbohydrate Surface of a Xenogenic Cell Substantially Reduces Human Antibody Binding and Complement-Mediated Cytolysis,” Nature Med., 1995, pp. 1261-1267, vol. 1.
Sandrin et al., “Reduction of the Major Porcine Xenoantigen Galα(1,3)Gal by Expression of α(1,2)fucosyltransferase,” Xenotransplantation, 1996, pp. 134-140, vol. 3.
Schreiner et al., “Essential Fatty Acid Depletion of Renal Allografts and Prevention of Rejection,” Science, 1988, pp. 1032-1033, vol. 240.
Tamponnet et al., “Rabbit Articular Chondrocytes in Alginate Gel, Characterization of Immobilized Preparations and Potential Applications,” Appl. Microbiol. Biotechnol., 1992, pp. 311-315, vol. 37.
Adkisson et al., “Unique Fatty Acid Composition of Normal Cartilage: Discovery of High Levels of n-9 Eicosatrienoic Acid and Low Levels of n-6 Polyunsaturated Fatty Acids,” FASEB J., 1991, pp. 344-353, vol. 5.
Ismaiel et al., “Differential Responses of Human and Rat Cartilage to Degrading Stimuli In-Vitro,” J. Pharm. Pharmacol., 1991, pp. 207-209, vol. 43.
Steinberg et al., “A Tissue Culture Model of Cartilage Breakdown in Rheumatoid Arthritis. III. Effects of Antirheumatic Drugs,” Arthritis & Rheumatism, 1979, pp. 877-885, vol. 22.

Affiliated with

Also associated with

Rating

Cartilage composites and methods of use does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cartilage composites and methods of use, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cartilage composites and methods of use will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3664753