Carrageeman-immobilized esterase

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

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435134, 435178, 435182, 435198, C12P 762, C12P 764, C12N 1110, C12N 920

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active

052623130

ABSTRACT:
Carrageenan-immobilized enzymes are prepared that are stable and retain high activity toward substrates when used in substantially water-immiscible organic solvents. The carrageenan-immobilized enzymes are preferably prepared by introducing droplets of an enzyme/carrageenan solution into a chilled alcohol saturated with a potassium salt and hardening the droplets in the alcohol. The alcohol is selected from n-butanol, benzyl alcohol, crotyl alcohol, n-propanol, isopropanol and sec-butanol. Reactions catalyzed by a .kappa.-carrageenan-immobilized esterase include steroselective transesterifications and hydrolysis reactions.

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