Carotenoid ketolase gene

Details Carotenoid ketolase gene Carotenoid ketolase gene

2006-01-10

2006-01-10

Patterson, Jr., Charles L. (Department: 1652)

Chemistry: molecular biology and microbiology

Plant cell or cell line, per se ; composition thereof;...

C435S252300, C435S254110, C435S252200, C435S193000, C536S023200

Reexamination Certificate

active

06984523

ABSTRACT:
A ketolase gene has been isolated fromRhodococcus erythropolisAN12 strain encoding a carotenoid modification enzyme of the carotenoid biosynthetic pathway. The gene and gene product are the first isolated from aRhodococcusstrain. Six conserved amino acid motifs have been identified as the characteristic of this type of ketolase enzymes. The gene and gene product of the present invention may be used in a variety of ways for the production of keto-carotenoid compounds in a variety of organisms.

REFERENCES:
Hannibal et al.,Isolation and Characterization of Canthaxanthin Biosynthesis Genes from the Photosynthetic BacteriumBrandyrhizobiumsp. Strain ORS278, J. Bacteriol.(2000) 182: 3850-3853.
Fernandez-Gonzalez et al., A New Type of Asymmetrically Acting β-Carotene Ketolase Is Required for the Synthesis of Echinenone in the CyanobacteriumSynechocystissp. PCC 6803, J.of Biol. Chem.(1997) 272;9728-9733.
Mann et al., Metabolic engineering of astaxanthin production in tobacco flowers, (2000) Nature Biotechnology, 18:888-892.
Ichiyama et al., Carotenoid Pigments of GenusRhodococcus, Microbiol.Immunol. (1989), 33:503-508.
White O et al., Genome Sequence of the Radioresistant BacteriumDeinococcus radioduransR1, Science 286 (5444), 1571-1577 (1999).
NCBI, Accession No. NC00091, Synechocystis CrtO(slr0088), Oct. 23, 2001.
NCBI, Accession No. AE001872,Deinococcusgene (DR0093), Nov. 22, 1999.

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