Carotenoid ketolase gene

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing a carotene nucleus

Reexamination Certificate

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C435S193000

Reexamination Certificate

active

07074588

ABSTRACT:
A ketolase gene has been isolated fromRhodococcus erythropolisAN12 strain encoding a carotenoid modification enzyme of the carotenoid biosynthetic pathway. The gene and gene product are the first isolated from aRhodococcusstrain. Six conserved amino acid motifs have been identified as the characteristic of this type of ketolase enzymes. The gene and gene product of the present invention may be used in a variety of ways for the production of keto-carotenoid compounds in a variety of organisms.

REFERENCES:
patent: 5750865 (1998-05-01), Bird et al.
patent: WO 02/18617 (2002-03-01), None
Hannibal et al., Isolation and Characterization of Canthaxanthin Biosynthesis Genes from the Photosynthetic Bacterium Brandyrhizobium sp. Strain ORS278, J. Bacteriol.(2000) 182: 3850-3853.
Fernandez-Gonzalez et al., A New Type of Asymmetrically Acting β-Carotene Ketolase Is Required for the Synthesis of Echinenone in the Cyanobacterium Synechocystis sp. PCC 6803, J.of Biol. Chem.(1997) 272;9728-9733.
Mann et al., Metabolic engineering of astaxanthin production in tobacco flowers, (2000) Nature Biotechnology, 18:888-892.
Ichiyama et al., Carotenoid Pigments of Genus Rhodococcus, Microbiol. Immunol. (1989), 33:503-508.
White O et al., Genome Sequence of the Radioresistant Bacterium Deinococcus radiodurans R1, Science 286 (5444), 1571-1577 (1999).
NCBI, Accession No. NC00091, Synechocystis CrtO(slr0088), Oct. 23, 2001.
NCBI, Accession No. AE001872, Deinococcus gene (DR0093), Nov. 22, 1999.
P.C. Lee et al., Metabolic Engineering Towards Biotechnological Production Of Carotenoids In Microorganisms, Appl. Microbiol. Biotechnol., 2000, pp. 1-11, vol. 60.

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