Carboxymethyl cellulase from Thermotoga maritima

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 232, 536 2432, C07H 2104, C07H 2102

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active

059257490

ABSTRACT:
A purified thermostable enzyme is derived from the eubacterium T. maritima. The enzyme has a molecular weight as determined by gel electrophoresis of about 35 kilodaltons and has cellulase activity. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of cellulose where desired.

REFERENCES:
patent: 5420029 (1995-05-01), Gelfand et al.
Accession Q32472 on the N-Geneseq database. Submitted by Hayashi et al. Apr. 26, 1993.
Shima, S. et al. "Nucleotide sequence analysis of the endo-glucanase-encoding gene, ceICCD, of Clostridium cellulolyticum." Gene (Jul. 1991), vol. 104, No. 1, pp. 33-38.
Ooi et al., Expression of the Cellulase (F1-CM Case) Gene . . . , Biosci. Biotech. Biochem 57(11):1960-1961 (1993).
Ooi et al., Expression of the Cellulase (F1-CM Case) Gene . . . , Biosci. Biotech. Biochem 58(5):954-956 (1994).
Gilbert et al., Evidence of Multiple Carboxymethyl Cellulase Genes . . . , Molecular Gen Genetics 210:551-556 (1987).
Bronnenmeier et al., Purification of Thermatoga Maritima Enzymes for the Degradation of Cellulosic Materials; Apr. 1995; vol. 61, No. 4; pp. 1399-1407.

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